PMID- 21728860
OWN - NLM
STAT- MEDLINE
DCOM- 20111021
LR  - 20131121
IS  - 1943-5681 (Electronic)
IS  - 0002-9645 (Linking)
VI  - 72
IP  - 7
DP  - 2011 Jul
TI  - In vitro and in vivo responses of mucosa from the large colon of horses to 
      ischemia and reperfusion.
PG  - 982-9
LID - 10.2460/ajvr.72.7.982 [doi]
AB  - OBJECTIVE: To induce ischemia and reperfusion injury in the large colon mucosa of 
      horses in vivo and evaluate the recovery and effects of components of an organ 
      transplant solution on mucosal recovery in vitro. ANIMALS: 6 healthy horses. 
      PROCEDURES: Horses were anesthetized, and ischemia was induced for 60 minutes in 
      the pelvic flexure, which was followed by reperfusion for 240 minutes. Ischemic 
      (n = 4 horses), reperfused (6), and adjacent control (6) colonic mucosae were 
      isolated for in vitro testing and histologic examinations. Tissues were mounted 
      in Ussing chambers with plain Krebs Ringer bicarbonate (KRB), KRB with 
      N-acetylcysteine (NAC), or KRB with a modified organ transplant solution (MOTS). 
      Transepithelial electrical resistance (TER) and mannitol flux were used to assess 
      mucosal integrity. Data were analyzed by use of ANOVA and Kruskal-Wallis tests. 
      RESULTS: The TER in reperfused tissues was similar to the TER in control tissues 
      and greater than the TER in ischemic tissues, which was consistent with 
      morphological evidence of recovery in reperfused tissues. Mannitol flux was 
      greater in ischemic tissues than in reperfused tissues. The TER and mannitol flux 
      were not significantly affected by incubation of mucosa with NAC or MOTS. 
      CONCLUSIONS AND CLINICAL RELEVANCE: Ischemia induced during the brief period 
      allowed rapid mucosal repair and complete recovery of tissue barrier properties 
      during reperfusion. Therefore, reperfusion injury was not observed for this 
      method of ischemic damage in equine colonic mucosa.
FAU - Graham, A Sarah
AU  - Graham AS
AD  - Island Whirl Equine Colic Research Laboratory, Department of Large Animal 
      Clinical Sciences, College of Veterinary Medicine, University of Florida, 
      Gainesville, FL 32610, USA. sarahgraham@ufl.edu
FAU - Grosche, Astrid
AU  - Grosche A
FAU - Morton, Alison J
AU  - Morton AJ
FAU - Polyak, Maximilian M R
AU  - Polyak MM
FAU - Freeman, David E
AU  - Freeman DE
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Am J Vet Res
JT  - American journal of veterinary research
JID - 0375011
RN  - 0 (Anesthetics, Intravenous)
RN  - 0 (Hypnotics and Sedatives)
RN  - 2KFG9TP5V8 (Xylazine)
RN  - 3OWL53L36A (Mannitol)
RN  - 690G0D6V8H (Ketamine)
RN  - Q3JTX2Q7TU (Diazepam)
SB  - IM
MH  - Analysis of Variance
MH  - Anesthetics, Intravenous/administration & dosage
MH  - Animals
MH  - Colon/blood supply/*pathology
MH  - Diazepam/administration & dosage
MH  - Electric Impedance
MH  - Horse Diseases/chemically induced/*pathology
MH  - Horses
MH  - Hypnotics and Sedatives/administration & dosage
MH  - Intestinal Mucosa/pathology
MH  - Ischemia/pathology/*veterinary
MH  - Ketamine/administration & dosage
MH  - Mannitol/metabolism
MH  - Reperfusion Injury/pathology/*veterinary
MH  - Statistics, Nonparametric
MH  - Xylazine/administration & dosage
EDAT- 2011/07/07 06:00
MHDA- 2011/10/22 06:00
CRDT- 2011/07/07 06:00
PHST- 2011/07/07 06:00 [entrez]
PHST- 2011/07/07 06:00 [pubmed]
PHST- 2011/10/22 06:00 [medline]
AID - 10.2460/ajvr.72.7.982 [doi]
PST - ppublish
SO  - Am J Vet Res. 2011 Jul;72(7):982-9. doi: 10.2460/ajvr.72.7.982.