PMID- 2172938
OWN - NLM
STAT- MEDLINE
DCOM- 19901212
LR  - 20190818
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 11
IP  - 4
DP  - 1990 Jul-Aug
TI  - Synthesis and biological activity of a biotinylated vasopressin analog.
PG  - 687-91
AB  - To study vasopressin receptor-mediated endocytosis using electronmicroscopy 
      methods and to develop avidin affinity columns for receptor purification, we 
      synthesized and tested the biological properties of a biotinylated vasopressin 
      (VP) analog [1-(2-mercapto) propionic acid] 8-[lysine-N6-biotin] VP (B-MLVP). 
      B-MLVP was prepared by coupling biotin to the epsilon amine of the lysine residue 
      in [1-(2-mercapto) propionic acid] 8-(lysine) VP (MLVP). The structure of HPLC 
      purified B-MLVP was confirmed by fast atom bombardment mass spectrometry. B-MLVP 
      effectively competed for arginine vasopressin (AVP) binding sites in canine renal 
      plasma membranes on the surface of LLC-PK1 kidney cells. Dissociation constants 
      of 15 nM and 202 nM were calculated from the results of competition binding 
      assays conducted with membranes and cells, respectively. B-MLVP stimulated 
      adenylate cyclase activity and elevated cellular 3',5',cyclic-AMP (cAMP) content 
      in a manner similar to AVP, indicating it is an agonist of VP action in renal 
      tissue. These observations indicate that B-MLVP is an agonist of VP action and 
      may be used to study renal VP receptors by employing avidin coupled to various 
      reporter groups.
FAU - Lutz, W H
AU  - Lutz WH
AD  - Department of Medicine, Mayo Clinic and Foundation, Rochester, MN 55905.
FAU - Londowski, J M
AU  - Londowski JM
FAU - Kumar, R
AU  - Kumar R
LA  - eng
GR  - R01 DK025409/DK/NIDDK NIH HHS/United States
GR  - DK-07013/DK/NIDDK NIH HHS/United States
GR  - DK-25409/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Receptors, Angiotensin)
RN  - 0 (Receptors, Vasopressin)
RN  - 130154-83-5 (vasopressin, ((2-mercapto)propionic acid)(1)-(Lys-N(6)-biotin)(8)-)
RN  - 50-57-7 (Lypressin)
RN  - 6SO6U10H04 (Biotin)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.6.1.1 (Adenylyl Cyclases)
SB  - IM
MH  - Adenylyl Cyclases/metabolism
MH  - *Amino Acid Sequence
MH  - Animals
MH  - Binding, Competitive/physiology
MH  - Biotin/*analogs & derivatives/chemical synthesis/metabolism/pharmacology
MH  - Cells, Cultured
MH  - Cyclic AMP/metabolism
MH  - Dogs
MH  - In Vitro Techniques
MH  - Kidney Medulla/metabolism
MH  - Lypressin/*analogs & derivatives/chemical synthesis/metabolism/pharmacology
MH  - Microscopy, Fluorescence
MH  - Molecular Sequence Data
MH  - Molecular Structure
MH  - Receptors, Angiotensin/metabolism
MH  - Receptors, Vasopressin
EDAT- 1990/07/01 00:00
MHDA- 1990/07/01 00:01
CRDT- 1990/07/01 00:00
PHST- 1990/07/01 00:00 [pubmed]
PHST- 1990/07/01 00:01 [medline]
PHST- 1990/07/01 00:00 [entrez]
AID - 0196-9781(90)90181-4 [pii]
AID - 10.1016/0196-9781(90)90181-4 [doi]
PST - ppublish
SO  - Peptides. 1990 Jul-Aug;11(4):687-91. doi: 10.1016/0196-9781(90)90181-4.