PMID- 21734728
OWN - NLM
STAT- MEDLINE
DCOM- 20120409
LR  - 20240323
IS  - 1751-7370 (Electronic)
IS  - 1751-7362 (Print)
IS  - 1751-7362 (Linking)
VI  - 6
IP  - 1
DP  - 2012 Jan
TI  - Expression patterns of mRNAs for methanotrophy and thiotrophy in symbionts of the 
      hydrothermal vent mussel Bathymodiolus puteoserpentis.
PG  - 104-12
LID - 10.1038/ismej.2011.81 [doi]
AB  - The hydrothermal vent mussel Bathymodiolus puteoserpentis (Mytilidae) from the 
      Mid-Atlantic Ridge hosts symbiotic sulfur- and methane-oxidizing bacteria in its 
      gills. In this study, we investigated the activity and distribution of these two 
      symbionts in juvenile mussels from the Logatchev hydrothermal vent field (14 degrees 45'N 
      Mid-Atlantic Ridge). Expression patterns of two key genes for chemosynthesis were 
      examined: pmoA (encoding subunit A of the particulate methane monooxygenase) as 
      an indicator for methanotrophy, and aprA (encoding the subunit A of the 
      dissimilatory adenosine-5'-phosphosulfate reductase) as an indicator for 
      thiotrophy. Using simultaneous fluorescence in situ hybridization (FISH) of rRNA 
      and mRNA we observed highest mRNA FISH signals toward the ciliated epithelium 
      where seawater enters the gills. The levels of mRNA expression differed between 
      individual specimens collected in a single grab from the same sampling site, 
      whereas no obvious differences in symbiont abundance or distribution were 
      observed. We propose that the symbionts respond to the steep temporal and spatial 
      gradients in methane, reduced sulfur compounds and oxygen by modifying gene 
      transcription, whereas changes in symbiont abundance and distribution take much 
      longer than regulation of mRNA expression and may only occur in response to 
      long-term changes in vent fluid geochemistry.
FAU - Wendeberg, Annelie
AU  - Wendeberg A
AD  - Department of Molecular Ecology, Celsiusstr, Max Planck Institute for Marine 
      Microbiology, Bremen, Germany. annelie.pernthaler@ufz.de
FAU - Zielinski, Frank U
AU  - Zielinski FU
FAU - Borowski, Christian
AU  - Borowski C
FAU - Dubilier, Nicole
AU  - Dubilier N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110707
PL  - England
TA  - ISME J
JT  - The ISME journal
JID - 101301086
RN  - 0 (RNA, Bacterial)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Ribosomal)
RN  - 0 (Sulfides)
RN  - OP0UW79H66 (Methane)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Gills/microbiology
MH  - Hydrothermal Vents/*microbiology
MH  - In Situ Hybridization, Fluorescence
MH  - Methane/metabolism
MH  - Methylococcaceae/*genetics/*metabolism
MH  - Mytilidae/*microbiology/physiology
MH  - Oxygen/metabolism
MH  - RNA Stability
MH  - RNA, Bacterial/analysis
MH  - RNA, Messenger/analysis
MH  - RNA, Ribosomal/analysis
MH  - Seawater/microbiology
MH  - Sulfides/metabolism
MH  - Symbiosis
MH  - Thiobacillus/genetics/metabolism
PMC - PMC3246237
EDAT- 2011/07/08 06:00
MHDA- 2012/04/10 06:00
PMCR- 2013/01/01
CRDT- 2011/07/08 06:00
PHST- 2011/07/08 06:00 [entrez]
PHST- 2011/07/08 06:00 [pubmed]
PHST- 2012/04/10 06:00 [medline]
PHST- 2013/01/01 00:00 [pmc-release]
AID - ismej201181 [pii]
AID - 10.1038/ismej.2011.81 [doi]
PST - ppublish
SO  - ISME J. 2012 Jan;6(1):104-12. doi: 10.1038/ismej.2011.81. Epub 2011 Jul 7.