PMID- 2173663
OWN - NLM
STAT- MEDLINE
DCOM- 19901228
LR  - 20151119
IS  - 0014-2980 (Print)
IS  - 0014-2980 (Linking)
VI  - 20
IP  - 10
DP  - 1990 Oct
TI  - Cross-talk between B cell surface immunoglobulin and interleukin 4 receptors: the 
      role of protein kinase C and Ca2(+)-mediated signals.
PG  - 2301-7
AB  - A well-known property of IL4 is its capacity to synergize with low concentrations 
      of anti-immunoglobulin (Ig) antibodies to induce B cells to synthesize DNA. 
      Cross-linking of surface Ig receptors stimulates phosphoinositide hydrolysis, 
      with consequent production of two signals: the elevation of intracellular Ca2+ 
      levels and activation of protein kinase C (PKC). Little is known about the second 
      messengers utilized by interleukin (IL)4 receptors. In this study we have 
      investigated the relative contributions of the two signals emanating from the 
      ligation of surface Ig receptors to the synergistic activation of B cells by IL4. 
      We show that IL4 plus carefully titrated concentrations of PKC-activating phorbol 
      esters [such as phorbol 12,13-dibutyrate (PBu2)] induce cell cycle entry of 
      virtually all murine B cells and substantial levels of DNA synthesis. Ca2+ 
      ionophores, in contrast do not act as co-mitogens with IL4. However, a critical 
      concentration of ionomycin further enhanced DNA synthesis induced by PBu2 plus 
      IL4. Taken together, these results suggest that PKC activation alone is 
      sufficient to synergize with IL4 in inducing B cells to enter cell cycle. 
      However, the co-mitogenic effects of anti-Ig and IL4 are evidently also dependent 
      on Ca2+ signals. This interpretation is supported by the findings that 
      cyclosporin, which abrogates the activation of lymphocytes by Ca2(+)-dependent 
      stimuli, inhibits B cell proliferation induced by anti-Ig plus IL4, but not the 
      response to PBu2 plus IL4.
FAU - Klaus, G G
AU  - Klaus GG
AD  - Division of Immunology, National Institute for Medical Research, Mill Hill, 
      London, GB.
FAU - Harnett, M M
AU  - Harnett MM
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Phosphatidylinositols)
RN  - 0 (Receptors, Antigen, B-Cell)
RN  - 0 (Receptors, Interleukin-4)
RN  - 0 (Receptors, Mitogen)
RN  - 37558-16-0 (Phorbol 12,13-Dibutyrate)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Antibodies, Monoclonal
MH  - B-Lymphocytes/enzymology/*immunology
MH  - Calcium/*metabolism
MH  - DNA/biosynthesis
MH  - Flow Cytometry
MH  - Lymphocyte Activation
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Phorbol 12,13-Dibutyrate
MH  - Phosphatidylinositols/metabolism
MH  - Protein Kinase C/*metabolism
MH  - Receptors, Antigen, B-Cell/*immunology
MH  - Receptors, Interleukin-4
MH  - Receptors, Mitogen/*immunology
MH  - Signal Transduction/*physiology
MH  - Tetradecanoylphorbol Acetate
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - 10.1002/eji.1830201020 [doi]
PST - ppublish
SO  - Eur J Immunol. 1990 Oct;20(10):2301-7. doi: 10.1002/eji.1830201020.