PMID- 21737754
OWN - NLM
STAT- MEDLINE
DCOM- 20120112
LR  - 20211020
IS  - 1539-7262 (Electronic)
IS  - 0022-2275 (Print)
IS  - 0022-2275 (Linking)
VI  - 52
IP  - 10
DP  - 2011 Oct
TI  - Quantification of liver fat in mice: comparing dual-echo Dixon imaging, chemical 
      shift imaging, and 1H-MR spectroscopy.
PG  - 1847-55
LID - 10.1194/jlr.D016691 [doi]
AB  - We evaluated dual-echo Dixon in-phase and out-of-phase (IP-OP), chemical shift 
      imaging (CSI), and (1)H MRS (hydrogen MR spectroscopy) in estimating fat content 
      (FC) in phantoms and in livers of mice. Phantoms were made according to the 
      volume percentage of fat ranging from 0% to 100%. The three MR methods were 
      performed to measure FC in phantoms and in livers of obese leptin-deficient 
      (ob/ob), human BSCL2/seipin gene knockout (SKO), and wild-type (WT) mice. The 
      results were compared with known FC in phantoms and to a reference standard from 
      mice by histological semiautomatic vacuole segmentation (HIS-S) procedure and 
      liver lipid (LL) chemical analysis. In phantoms, CSI underestimated FC from 50% 
      to 100%, to a lesser extent than IP-OP. In vivo, liver FC in ob/ob and SKO mice 
      measured by the three MR methods were all significantly higher than that in WT 
      mice. Liver FC measured by IP-OP were significantly lower than that measured by 
      CSI and MRS, with no significant difference between CSI and MRS. CSI and MRS 
      showed a linear correlation with LL analysis and with each other. IP-OP 
      underestimated FC, whereas CSI and MRS were more accurate for quantifying FC in 
      both phantoms and liver. CSI and MRS have the potential to replace HIS-S and LL 
      analysis in longitudinal studies.
FAU - Peng, Xin-Gui
AU  - Peng XG
AD  - Laboratory of Molecular Imaging, Department of Radiology, Zhongda Hospital, 
      Southeast University, Nanjing 210009, China.
FAU - Ju, Shenghong
AU  - Ju S
FAU - Qin, Yujiao
AU  - Qin Y
FAU - Fang, Fang
AU  - Fang F
FAU - Cui, Xin
AU  - Cui X
FAU - Liu, George
AU  - Liu G
FAU - Ni, Yicheng
AU  - Ni Y
FAU - Teng, Gao-Jun
AU  - Teng GJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110707
PL  - United States
TA  - J Lipid Res
JT  - Journal of lipid research
JID - 0376606
RN  - 0 (Fats)
SB  - IM
MH  - Animals
MH  - Echo-Planar Imaging/*methods
MH  - Fats/*analysis/chemistry/metabolism
MH  - Fatty Liver/metabolism/pathology
MH  - Humans
MH  - Liver/chemistry/*diagnostic imaging/metabolism/*pathology
MH  - Magnetic Resonance Imaging/*methods
MH  - Magnetic Resonance Spectroscopy/*methods
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Models, Animal
MH  - Non-alcoholic Fatty Liver Disease
MH  - Obesity/metabolism/pathology
MH  - Radiography
PMC - PMC3172999
EDAT- 2011/07/09 06:00
MHDA- 2012/01/13 06:00
PMCR- 2012/10/01
CRDT- 2011/07/09 06:00
PHST- 2011/07/09 06:00 [entrez]
PHST- 2011/07/09 06:00 [pubmed]
PHST- 2012/01/13 06:00 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - S0022-2275(20)40849-1 [pii]
AID - d016691 [pii]
AID - 10.1194/jlr.D016691 [doi]
PST - ppublish
SO  - J Lipid Res. 2011 Oct;52(10):1847-55. doi: 10.1194/jlr.D016691. Epub 2011 Jul 7.