PMID- 21737937
OWN - NLM
STAT- MEDLINE
DCOM- 20111128
LR  - 20131121
IS  - 1347-6947 (Electronic)
IS  - 0916-8451 (Linking)
VI  - 75
IP  - 7
DP  - 2011
TI  - Enrichment and characterization of a trichloroethene-dechlorinating consortium 
      containing multiple "dehalococcoides" strains.
PG  - 1268-74
AB  - A microbial consortium that reductively dechlorinates trichloroethene, 
      cis-1,2-dichloroethene (cis-DCE), and vinyl chloride (VC) to ethene with 
      methanogenesis was enriched from chloroethene-contaminated soil from Japan. 
      Dechlorination activity was maintained for over 4 years. Using quantitative 
      polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis 
      (DGGE) analysis targeting the "Dehalococcoides" 16S rRNA gene, four strains were 
      detected. Their growth and dechlorination activities were classified into two 
      types: one that grows by converting cis-DCE to ethene and the other that grows by 
      converting cis-DCE to VC. Then, the vcrA and bvcA genes encoding cis-DCE/VC 
      reductive dehalogenases were detected. Inhibitors of methanogenesis 
      (2-bromoethanesulfonate) and sulfidogenesis (molybdate) led to accumulation of 
      cis-DCE and of VC respectively. These results suggest that methanogens and 
      sulfate-reducing bacteria can play a significant role in dechlorination by 
      "Dehalococcoides."
FAU - Futagami, Taiki
AU  - Futagami T
AD  - Department of Bioscience and Biotechnology, Faculty of Agriculture, Kyushu 
      University, Hakozaki, Fukuoka, Japan.
FAU - Okamoto, Fuyuki
AU  - Okamoto F
FAU - Hashimoto, Hiroshi
AU  - Hashimoto H
FAU - Fukuzawa, Kotaro
AU  - Fukuzawa K
FAU - Higashi, Koji
AU  - Higashi K
FAU - Nazir, K H M Nazmul Hussain
AU  - Nazir KH
FAU - Wada, Erina
AU  - Wada E
FAU - Suyama, Akiko
AU  - Suyama A
FAU - Takegawa, Kaoru
AU  - Takegawa K
FAU - Goto, Masatoshi
AU  - Goto M
FAU - Nakamura, Kanji
AU  - Nakamura K
FAU - Furukawa, Kensuke
AU  - Furukawa K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110707
PL  - England
TA  - Biosci Biotechnol Biochem
JT  - Bioscience, biotechnology, and biochemistry
JID - 9205717
RN  - 0 (Ethylenes)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 290YE8AR51 (Trichloroethylene)
RN  - 91GW059KN7 (ethylene)
RN  - OP0UW79H66 (Methane)
RN  - WD06X94M2D (Vinyl Chloride)
SB  - IM
MH  - Biodegradation, Environmental
MH  - Chloroflexi/classification/genetics/*growth & development
MH  - Denaturing Gradient Gel Electrophoresis/methods
MH  - Ethylenes/analysis/metabolism
MH  - Halogenation/physiology
MH  - Methane/analysis/metabolism
MH  - Methanomicrobiaceae/growth & development
MH  - *Microbial Consortia
MH  - RNA, Ribosomal, 16S/*genetics
MH  - Soil Microbiology
MH  - Species Specificity
MH  - Trichloroethylene/*analysis/metabolism
MH  - Vinyl Chloride/analysis/metabolism
EDAT- 2011/07/09 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/07/09 06:00
PHST- 2011/07/09 06:00 [entrez]
PHST- 2011/07/09 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - JST.JSTAGE/bbb/110028 [pii]
AID - 10.1271/bbb.110028 [doi]
PST - ppublish
SO  - Biosci Biotechnol Biochem. 2011;75(7):1268-74. doi: 10.1271/bbb.110028. Epub 2011 
      Jul 7.