PMID- 21739990
OWN - NLM
STAT- MEDLINE
DCOM- 20111227
LR  - 20131121
IS  - 1936-086X (Electronic)
IS  - 1936-0851 (Linking)
VI  - 5
IP  - 8
DP  - 2011 Aug 23
TI  - Target-specific gene silencing of layer-by-layer assembled 
      gold-cysteamine/siRNA/PEI/HA nanocomplex.
PG  - 6138-47
LID - 10.1021/nn2017793 [doi]
AB  - Target-specific intracellular delivery of small interfering RNA (siRNA) is 
      regarded as one of the most important technologies for the development of siRNA 
      therapeutics. In this work, a cysteamine modified gold nanoparticles 
      (AuCM)/siRNA/polyethyleneimine (PEI)/hyaluronic acid (HA) complex was 
      successfully developed using a layer-by-layer method for target-specific 
      intracellular delivery of siRNA by HA receptor mediated endocytosis. Atomic force 
      microscopic and zeta potential analyses confirmed the formation of a 
      AuCM/siRNA/PEI/HA complex having a particle size of ca. 37.3 nm and a negative 
      surface charge of ca. -12 mV. With a negligible cytotoxicity, AuCM/siRNA/PEI/HA 
      complex showed an excellent target-specific gene silencing efficiency of ca. 70% 
      in the presence of 50 vol % serum, which was statistically much higher than that 
      of siRNA/Lipofectamine 2000 complex. In the competitive binding tests with free 
      HA, dark-field bioimaging and inductively coupled plasma-atomic emission 
      spectroscopy confirmed the target-specific intracellular delivery of 
      AuCM/siRNA/PEI/HA complex to B16F1 cells with HA receptors. Moreover, the 
      systemic delivery of AuCM/siRNA/PEI/HA complex using apolipoprotein B (ApoB) 
      siRNA as a model drug resulted in a significantly reduced ApoB mRNA level in the 
      liver tissue. Taken together, AuCM/siRNA/PEI/HA complex was thought to be 
      developed as target-specific siRNA therapeutics for the systemic treatment of 
      various liver diseases.
CI  - (c) 2011 American Chemical Society
FAU - Lee, Min-Young
AU  - Lee MY
AD  - Department of Materials Science and Engineering, Pohang University of Science and 
      Technology (POSTECH), San 31, Hyoja-dong, Nam-gu, Pohang, Kyungbuk 790-784, 
      Korea.
FAU - Park, Sang-Jun
AU  - Park SJ
FAU - Park, Kitae
AU  - Park K
FAU - Kim, Ki Su
AU  - Kim KS
FAU - Lee, Hwiwon
AU  - Lee H
FAU - Hahn, Sei Kwang
AU  - Hahn SK
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110708
PL  - United States
TA  - ACS Nano
JT  - ACS nano
JID - 101313589
RN  - 0 (Apolipoproteins B)
RN  - 0 (Drug Carriers)
RN  - 0 (RNA, Small Interfering)
RN  - 5UX2SD1KE2 (Cysteamine)
RN  - 7440-57-5 (Gold)
RN  - 9002-98-6 (Polyethyleneimine)
RN  - 9004-61-9 (Hyaluronic Acid)
SB  - IM
MH  - Animals
MH  - Apolipoproteins B/deficiency/genetics
MH  - Base Sequence
MH  - Cell Line, Tumor
MH  - Cysteamine/*chemistry
MH  - Drug Carriers/chemistry/metabolism
MH  - Endocytosis
MH  - *Gene Silencing
MH  - Gold/*chemistry
MH  - Hyaluronic Acid/*chemistry
MH  - Intracellular Space/metabolism
MH  - Liver Diseases/genetics/metabolism/therapy
MH  - Mice
MH  - Models, Molecular
MH  - Molecular Conformation
MH  - Molecular Imaging
MH  - Nanostructures/chemistry
MH  - Nanotechnology/*methods
MH  - Polyethyleneimine/*chemistry
MH  - RNA, Small Interfering/*chemistry/genetics
EDAT- 2011/07/12 06:00
MHDA- 2011/12/28 06:00
CRDT- 2011/07/12 06:00
PHST- 2011/07/12 06:00 [entrez]
PHST- 2011/07/12 06:00 [pubmed]
PHST- 2011/12/28 06:00 [medline]
AID - 10.1021/nn2017793 [doi]
PST - ppublish
SO  - ACS Nano. 2011 Aug 23;5(8):6138-47. doi: 10.1021/nn2017793. Epub 2011 Jul 8.