PMID- 2174137
OWN - NLM
STAT- MEDLINE
DCOM- 19910110
LR  - 20170214
IS  - 0194-5998 (Print)
IS  - 0194-5998 (Linking)
VI  - 103
IP  - 4
DP  - 1990 Oct
TI  - First place--Resident Basic Science Award 1990. Fibronectin-laminin combination 
      enhances peripheral nerve regeneration across long gaps.
PG  - 509-18
AB  - An exogenous fibronectin-laminin (FN-LAM) solution was added into silicone 
      chambers to determine the effects on peripheral nerve regeneration across 18-mm 
      long gaps. The sciatic nerves of adult rats were sutured into silicone chambers 
      20 mm in length, creating an 18-mm gap between the proximal and distal nerve 
      stumps. The chambers were filled with either a mixture of fibronectin and laminin 
      (500 micrograms/ml each) or a solution of cytochrome C (1 mg/ml) as the control. 
      After six weeks, the animals were killed and the chambers were examined for 
      regeneration. Seventy percent of the animals from the FN-LAM group demonstrated 
      regeneration across the 18-mm gaps, compared to only 30% in the control group. 
      The combination of FN-LAM significantly increased the number of axons that grew 
      into the distal end of the chamber (FN-LAM, 1325 +/- 522; cytochrome C, 153 +/- 
      104; p = 0.03). Examination of the distal tributaries of the sciatic nerve 
      revealed axons only in the FN-LAM group; none were found in the control group. 
      Quantitative analysis of neurons, retrogradely labeled with horseradish 
      peroxidase via injection of the sciatic nerve distal to the regenerated segment, 
      revealed a greater number of sensory and motor neurons in the FN-LAM group 
      compared to the control group. Morphometric studies revealed that the mean area 
      of the regenerated segment in the FN-LAM group was 37% larger than the controls, 
      and ultrastructural analysis demonstrated a more mature regenerated nerve. This 
      is the first in vivo demonstration that this combination of fibronectin and 
      laminin significantly enhances the regeneration of myelinated axons across a long 
      nerve gap in the rat sciatic nerve.
FAU - Woolley, A L
AU  - Woolley AL
AD  - Department of Otolaryngology, Washington University School of Medicine, St. 
      Louis, MO 63110.
FAU - Hollowell, J P
AU  - Hollowell JP
FAU - Rich, K M
AU  - Rich KM
LA  - eng
GR  - 5T32NS075-11/NS/NINDS NIH HHS/United States
GR  - HL20604/HL/NHLBI NIH HHS/United States
GR  - NS18071/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Otolaryngol Head Neck Surg
JT  - Otolaryngology--head and neck surgery : official journal of American Academy of 
      Otolaryngology-Head and Neck Surgery
JID - 8508176
RN  - 0 (Cytochrome c Group)
RN  - 0 (Fibronectins)
RN  - 0 (Laminin)
SB  - IM
MH  - Academies and Institutes
MH  - Animals
MH  - Awards and Prizes
MH  - Axons/drug effects
MH  - Cytochrome c Group/pharmacology
MH  - Female
MH  - Fibronectins/*pharmacology
MH  - Laminin/*pharmacology
MH  - Myelin Sheath/drug effects
MH  - Nerve Regeneration/*drug effects
MH  - Neurosurgery
MH  - Rats
MH  - Rats, Inbred Strains
MH  - Sciatic Nerve/cytology/drug effects
MH  - United States
EDAT- 1990/10/01 00:00
MHDA- 1990/10/01 00:01
CRDT- 1990/10/01 00:00
PHST- 1990/10/01 00:00 [pubmed]
PHST- 1990/10/01 00:01 [medline]
PHST- 1990/10/01 00:00 [entrez]
AID - 10.1177/019459989010300401 [doi]
PST - ppublish
SO  - Otolaryngol Head Neck Surg. 1990 Oct;103(4):509-18. doi: 
      10.1177/019459989010300401.