PMID- 21742111
OWN - NLM
STAT- MEDLINE
DCOM- 20111111
LR  - 20191210
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 700
IP  - 1-2
DP  - 2011 Aug 26
TI  - Validation study on avermectine residues in foodstuffs.
PG  - 11-5
LID - 10.1016/j.aca.2010.12.035 [doi]
AB  - Avermectines are antiparasitic agents widely used as veterinary drugs for food 
      producing animals. The European Community, due to their side effects, limited the 
      use of these molecules establishing maximum residue limits (MRLs) in some foods. 
      A validated qualitative and quantitative high performance liquid chromatography 
      method with fluorescence detection (HPLC-FL) is presented for the simultaneous 
      determination of ivermectin (IVM), abemectin (ABA), moxidectin (MOX), 
      eprinomectin (EPR), doramectin (DOR) and emamectin (EMA) in foodstuffs (muscle, 
      eggs and milk). Samples were extracted with acetonitrile, purified with 
      liquid-liquid extraction (LLE), and analysed by HLPC-FL previous derivatization 
      with trifluoroacetic anhydride (TFAA) in presence of 1-methyl-imidazole (MI) and 
      acetic acid. To date, the presented method is the first validated for the matrix 
      eggs, and in accordance with the requirements set by Commission Decision 
      2002/657/EC. Recoveries of the methods, calculated spiking the samples in the 
      range 5.0-100.0 mug kg(-1), were 64-83% for muscle, 65-89% for milk and 63-84% for 
      eggs. The precision (CV) ranged between 9.2 and 17.1% for muscle, 9.9 and 16.6% 
      for milk and from 9.4 to 17.4% for eggs. Linearity for the six analytes was 
      calculated from 5.0 to 200.0 mug kg(-1). The main advantages of the presented 
      method are its rapidity, the specificity, the good precision and recovery that 
      make it very suitable to the detection and determination of avermectines.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Giannetti, L
AU  - Giannetti L
AD  - Istituto Zooprofilattico Sperimentale Regioni Lazio e Toscana, Rome, Italy. 
      luigi.giannetti@izslt.it
FAU - Giorgi, A
AU  - Giorgi A
FAU - Necci, F
AU  - Necci F
FAU - Ferretti, G
AU  - Ferretti G
FAU - Buiarelli, F
AU  - Buiarelli F
FAU - Neri, B
AU  - Neri B
LA  - eng
PT  - Journal Article
PT  - Validation Study
DEP - 20110113
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Acetic Anhydrides)
RN  - 0 (Acetonitriles)
RN  - 0 (Antiparasitic Agents)
RN  - 0 (Fluoroacetates)
RN  - 5ENA87IZHT (trifluoroacetic anhydride)
RN  - E5R8Z4G708 (Trifluoroacetic Acid)
RN  - Z072SB282N (acetonitrile)
SB  - IM
MH  - Acetic Anhydrides
MH  - Acetonitriles/chemistry
MH  - Animals
MH  - Antiparasitic Agents/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Eggs/analysis
MH  - Fluoroacetates
MH  - Food Analysis/*methods
MH  - Milk/chemistry
MH  - Muscles/chemistry
MH  - Spectrometry, Fluorescence/methods
MH  - Trifluoroacetic Acid/chemistry
EDAT- 2011/07/12 06:00
MHDA- 2011/11/15 06:00
CRDT- 2011/07/12 06:00
PHST- 2010/10/01 00:00 [received]
PHST- 2010/12/13 00:00 [revised]
PHST- 2010/12/22 00:00 [accepted]
PHST- 2011/07/12 06:00 [entrez]
PHST- 2011/07/12 06:00 [pubmed]
PHST- 2011/11/15 06:00 [medline]
AID - S0003-2670(11)00057-2 [pii]
AID - 10.1016/j.aca.2010.12.035 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2011 Aug 26;700(1-2):11-5. doi: 10.1016/j.aca.2010.12.035. Epub 
      2011 Jan 13.