PMID- 21742126
OWN - NLM
STAT- MEDLINE
DCOM- 20111111
LR  - 20131121
IS  - 1873-4324 (Electronic)
IS  - 0003-2670 (Linking)
VI  - 700
IP  - 1-2
DP  - 2011 Aug 26
TI  - Determination of MRL regulated corticosteroids in liver from various species 
      using ultra high performance liquid chromatography-tandem mass spectrometry 
      (UHPLC).
PG  - 137-43
LID - 10.1016/j.aca.2010.12.042 [doi]
AB  - Dexamethasone, betamethasone, prednisolone and methylprednisolone are 
      corticosteroids widely used in animal husbandry. These compounds are licensed for 
      therapy in veterinary practices while their use for growth promoting practices, 
      mainly in combination with other growth promoters, is prohibited within the 
      European Union. In order to protect the consumer, maximum residue limits (MRLs) 
      have been set by the European Community in liver to 2.0 mug kg(-1) (dexamethasone 
      and betamethasone) and 10.0 mug kg(-1) (prednisolone and methylprednisolone) for 
      different species. The major challenges in the analysis of dexamethasone and 
      betamethasone consist in performing an efficient separation of both isomers and 
      in detecting and identifying all the molecules according to the regulatory 
      requirements fixed in Commission Decision 2002/657/EC. In this context, an 
      UHPLC-MS/MS method with a short runtime (7 min) and using the SRM acquisition 
      mode was developed and validated. An efficient selectivity of the sample 
      preparation combined with a high sensitivity of the measurement system allowed 
      identifying and quantifying the four corticosteroids of interest in this complex 
      biological matrix. Signals obtained were found very repeatable, even at very low 
      concentration levels with an unambiguous identification of the compounds. The 
      performance limits of the method have been validated according to the regulatory 
      requirements and the method has been successfully applied to the confirmation of 
      incurred liver samples.
CI  - Copyright (c) 2011 Elsevier B.V. All rights reserved.
FAU - Deceuninck, Yoann
AU  - Deceuninck Y
AD  - ONIRIS, Ecole nationale veterinaire, agroalimentaire et de l'alimentation 
      Nantes-Atlantique, Laboratoire d'Etude des Residus et Contaminants dans les 
      Aliments (LABERCA), Atlanpole-La Chantrerie, BP 40706, Nantes, France. 
      laberca@oniris-nantes.fr
FAU - Bichon, Emmanuelle
AU  - Bichon E
FAU - Monteau, Fabrice
AU  - Monteau F
FAU - Antignac, Jean-Philippe
AU  - Antignac JP
FAU - Le Bizec, Bruno
AU  - Le Bizec B
LA  - eng
PT  - Journal Article
DEP - 20110114
PL  - Netherlands
TA  - Anal Chim Acta
JT  - Analytica chimica acta
JID - 0370534
RN  - 0 (Adrenal Cortex Hormones)
RN  - 0 (Veterinary Drugs)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - 9842X06Q6M (Betamethasone)
RN  - 9PHQ9Y1OLM (Prednisolone)
RN  - X4W7ZR7023 (Methylprednisolone)
SB  - IM
MH  - Adrenal Cortex Hormones/*analysis
MH  - Animals
MH  - Betamethasone/analysis
MH  - Cattle
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Dexamethasone/analysis
MH  - Liver/*chemistry
MH  - Methylprednisolone/analysis
MH  - Prednisolone/analysis
MH  - Sheep
MH  - Swine
MH  - Tandem Mass Spectrometry/*methods
MH  - Veterinary Drugs/analysis
EDAT- 2011/07/12 06:00
MHDA- 2011/11/15 06:00
CRDT- 2011/07/12 06:00
PHST- 2010/08/31 00:00 [received]
PHST- 2010/12/11 00:00 [revised]
PHST- 2010/12/28 00:00 [accepted]
PHST- 2011/07/12 06:00 [entrez]
PHST- 2011/07/12 06:00 [pubmed]
PHST- 2011/11/15 06:00 [medline]
AID - S0003-2670(11)00067-5 [pii]
AID - 10.1016/j.aca.2010.12.042 [doi]
PST - ppublish
SO  - Anal Chim Acta. 2011 Aug 26;700(1-2):137-43. doi: 10.1016/j.aca.2010.12.042. Epub 
      2011 Jan 14.