PMID- 21742759
OWN - NLM
STAT- MEDLINE
DCOM- 20111209
LR  - 20211020
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Print)
IS  - 0305-1048 (Linking)
VI  - 39
IP  - 18
DP  - 2011 Oct
TI  - Fluorescence protection assay: a novel homogeneous assay platform toward 
      development of aptamer sensors for protein detection.
PG  - e122
LID - 10.1093/nar/gkr559 [doi]
AB  - Development of novel aptamer sensor strategies for rapid and selective assays of 
      protein biomarkers plays crucial roles in proteomics and clinical diagnostics. 
      Herein, we have developed a novel aptamer sensor strategy for homogeneous 
      detection of protein targets based on fluorescence protection assay. This 
      strategy is based on our reasoning that interaction of aptamer with its protein 
      target may dramatically increase steric hindrance, which protects the 
      fluorophore, fluorescein isothiocyannate (FITC), labeled at the binding pocket 
      from accessing and quenching by the FITC antibody. The aptamer sensor strategy is 
      demonstrated using a model protein target of immunoglobulin E (IgE), a known 
      biomarker associated with atopic allergic diseases. The results reveal that the 
      aptamer sensor shows substantial (>6-fold) fluorescence enhancement in response 
      to the protein target, thereby verifying the mechanism of fluorescence 
      protection. Moreover, the aptamer sensor displays improved specificity to other 
      co-existing proteins and a desirable dynamic range within the IgE concentration 
      from 0.1 to 50 nM with a readily achieved detection limit of 0.1 nM. Because of 
      great robustness, easy operation and scalability for parallel assays, the 
      developed homogeneous fluorescence protection assay strategy might create a new 
      methodology for developing aptamer sensors in sensitive, selective detection of 
      proteins.
FAU - Wang, Hong-Qi
AU  - Wang HQ
AD  - State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry 
      and Chemical Engineering, Hunan University, Changsha, 410082, P R China.
FAU - Wu, Zhan
AU  - Wu Z
FAU - Tang, Li-Juan
AU  - Tang LJ
FAU - Yu, Ru-Qin
AU  - Yu RQ
FAU - Jiang, Jian-Hui
AU  - Jiang JH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110708
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Aptamers, Nucleotide/*chemistry
MH  - *Biosensing Techniques
MH  - Fluorescent Dyes
MH  - Fluorometry/*methods
MH  - Immunoglobulin E/analysis
MH  - Proteins/*analysis
PMC - PMC3185441
EDAT- 2011/07/12 06:00
MHDA- 2011/12/14 06:00
PMCR- 2011/07/08
CRDT- 2011/07/12 06:00
PHST- 2011/07/12 06:00 [entrez]
PHST- 2011/07/12 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
PHST- 2011/07/08 00:00 [pmc-release]
AID - gkr559 [pii]
AID - 10.1093/nar/gkr559 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 2011 Oct;39(18):e122. doi: 10.1093/nar/gkr559. Epub 2011 Jul 
      8.