PMID- 21744465
OWN - NLM
STAT- MEDLINE
DCOM- 20120801
LR  - 20131121
IS  - 1522-7243 (Electronic)
IS  - 1522-7235 (Linking)
VI  - 27
IP  - 1
DP  - 2012 Jan-Feb
TI  - Determination of 5-hydroxytryptamine in serum by electrochemiluminescence 
      detection with the aid of capillary electrophoresis.
PG  - 63-8
LID - 10.1002/bio.1326 [doi]
AB  - A rapid, sensitive and simple electrochemiluminescence method for the 
      determination of 5-hydroxytryptamine (5-HT) using capillary electrophoresis was 
      proposed. The experimental parameters, including the detection potential, the 
      concentration of Ru(bpy)(3)(2+), the concentration and pH of phosphate buffer for 
      separation and detection, the injection voltage and time and the separation 
      voltage on the determination of 5-HT, were optimized. Under the optimized 
      conditions, the linear concentration range for 5-HT was 3.5 x 10(-9) -5.1 x 
      10(-3) mol/L, with a detection limit of 5 x 10(-10) mol/L. The relative standard 
      deviations (RSDs) of the ECL intensity and the migration times for six continuous 
      injections of 1.0 micromol/L 5-HT were 2.48% and 1.3%, respectively. The method was 
      successfully applied to 5-HT assay in samples of human serum in 5 min and the 
      extraction recoveries with spiked serum samples were over 94.4%.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Hu, Yuefang
AU  - Hu Y
AD  - State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, First 
      Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, 310003, 
      People's Republic of China.
FAU - Xu, Wei
AU  - Xu W
FAU - Li, Jianping
AU  - Li J
FAU - Li, Lanjuan
AU  - Li L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110711
PL  - England
TA  - Luminescence
JT  - Luminescence : the journal of biological and chemical luminescence
JID - 100889025
RN  - 333DO1RDJY (Serotonin)
RN  - 7UI0TKC3U5 (Ruthenium)
SB  - IM
MH  - Electrophoresis, Capillary/*methods
MH  - Humans
MH  - Limit of Detection
MH  - Luminescent Measurements/*methods
MH  - Ruthenium/chemistry
MH  - Serotonin/*blood
EDAT- 2011/07/12 06:00
MHDA- 2012/08/02 06:00
CRDT- 2011/07/12 06:00
PHST- 2011/03/20 00:00 [received]
PHST- 2011/05/02 00:00 [revised]
PHST- 2011/05/05 00:00 [accepted]
PHST- 2011/07/12 06:00 [entrez]
PHST- 2011/07/12 06:00 [pubmed]
PHST- 2012/08/02 06:00 [medline]
AID - 10.1002/bio.1326 [doi]
PST - ppublish
SO  - Luminescence. 2012 Jan-Feb;27(1):63-8. doi: 10.1002/bio.1326. Epub 2011 Jul 11.