PMID- 21745111
OWN - NLM
STAT- MEDLINE
DCOM- 20120529
LR  - 20220310
IS  - 1937-335X (Electronic)
IS  - 1937-3341 (Print)
IS  - 1937-3341 (Linking)
VI  - 17
IP  - 21-22
DP  - 2011 Nov
TI  - Effect of cell seeding density on proliferation and osteodifferentiation of 
      umbilical cord stem cells on calcium phosphate cement-fiber scaffold.
PG  - 2603-13
LID - 10.1089/ten.tea.2011.0048 [doi]
AB  - Calcium phosphate cement (CPC) can fill complex-shaped bone defects and set in 
      situ to form a scaffold with intimate adaptation to neighboring bone. The 
      objectives of this study were to determine (1) the effects of fiber length and 
      alginate microbead volume fraction on CPC mechanical properties, and (2) the 
      effect of cell seeding density of human umbilical cord mesenchymal stem cells 
      (hUCMSCs) on their proliferation and osteodifferentiation on CPC. Adding 
      microbeads to CPC degraded the strength. However, increasing the fiber length 
      improved the mechanical properties. Strength and elastic modulus of 
      CPC-microbead-fiber scaffold matched those reported for cancellous bone. When the 
      cell seeding density was increased from 50k to 300k, the cell viability, 
      osteodifferentiation, and bone mineral synthesis also increased. When the seeding 
      density was further increased to 500k, the osteodifferentiation and 
      mineralization decreased. Hence, the 300k seeding density was optimal for 
      CPC-microbead-fiber under the specified conditions. At day 8, alkaline 
      phosphatase (ALP) gene expression of hUCMSCs with seeding density of 300k was 
      threefold the ALP at 150k, and 200-fold the ALP at 50k. At day 14, osteocalcin 
      and runt-related transcription factor 2 with cell seeding density of 300k was 
      fourfold those at 50k. At day 14, mineralization by hUCMSCs at seeding density of 
      300k was 5-fold the mineralization at 150k, and 25-fold that at 50k. In 
      conclusion, the effect of stem cell seeding density on CPC was determined for the 
      first time. At low cell densities, cell viability and mineralization increased 
      with seeding density. However, a higher seeding density was not necessarily 
      better, and an optimal seeding density on CPC resulted in the best 
      osteodifferentiation and mineralization. The stem cell-seeded CPC-fiber scaffold 
      with excellent osteodifferentiation and mineralization is promising for 
      orthopedic and craniofacial applications.
FAU - Zhou, Hongzhi
AU  - Zhou H
AD  - Department of Endodontics, Prosthodontics, and Operative Dentistry, Biomaterials 
      and Tissue Engineering Division, University of Maryland Dental School, Baltimore, 
      Maryland 21201, USA.
FAU - Weir, Michael D
AU  - Weir MD
FAU - Xu, Hockin H K
AU  - Xu HH
LA  - eng
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - DE14190/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110711
PL  - United States
TA  - Tissue Eng Part A
JT  - Tissue engineering. Part A
JID - 101466659
RN  - 0 (Calcium Phosphates)
RN  - 97Z1WI3NDX (calcium phosphate)
SB  - IM
MH  - Biomechanical Phenomena
MH  - Calcium Phosphates/*chemistry
MH  - Cell Differentiation/physiology
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Humans
MH  - Osteogenesis/physiology
MH  - Stem Cells/*cytology/metabolism
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/*cytology
PMC - PMC3204200
EDAT- 2011/07/13 06:00
MHDA- 2012/05/30 06:00
PMCR- 2012/11/01
CRDT- 2011/07/13 06:00
PHST- 2011/07/13 06:00 [entrez]
PHST- 2011/07/13 06:00 [pubmed]
PHST- 2012/05/30 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - 10.1089/ten.tea.2011.0048 [pii]
AID - 10.1089/ten.tea.2011.0048 [doi]
PST - ppublish
SO  - Tissue Eng Part A. 2011 Nov;17(21-22):2603-13. doi: 10.1089/ten.tea.2011.0048. 
      Epub 2011 Jul 11.