PMID- 21746787
OWN - NLM
STAT- MEDLINE
DCOM- 20120524
LR  - 20211020
IS  - 1469-7793 (Electronic)
IS  - 0022-3751 (Print)
IS  - 0022-3751 (Linking)
VI  - 589
IP  - Pt 16
DP  - 2011 Aug 15
TI  - The influence of carbohydrate-protein co-ingestion following endurance exercise 
      on myofibrillar and mitochondrial protein synthesis.
PG  - 4011-25
LID - 10.1113/jphysiol.2011.211888 [doi]
AB  - The aim of the present study was to determine mitochondrial and myofibrillar 
      muscle protein synthesis (MPS) when carbohydrate (CHO) or carbohydrate plus 
      protein (C+P) beverages were ingested following prolonged cycling exercise. The 
      intracellular mechanisms thought to regulate MPS were also investigated. In a 
      single-blind, cross-over study, 10 trained cyclists (age 29 +/- 6 years, VO2max 
      66.5 +/- 5.1 ml kg(-1) min(-1)) completed two trials in a randomized order. 
      Subjects cycled for 90 min at 77 +/- 1% VO2max before ingesting a CHO (25 g of 
      carbohydrate) or C+P (25 g carbohydrate + 10 g whey protein) beverage immediately 
      and 30 min post-exercise. A primed constant infusion of 
      L-[ring-(13)C6]phenylalanine began 1.5 h prior to exercise and continued until 4 
      h post-exercise. Muscle biopsy samples were obtained to determine myofibrillar 
      and mitochondrial MPS and the phosphorylation of intracellular signalling 
      proteins. Arterialized blood samples were obtained throughout the protocol. 
      Plasma amino acid and urea concentrations increased following ingestion of C+P 
      only. Serum insulin concentration increased more for C+P than CHO. Myofibrillar 
      MPS was  approximately 35% greater for C+P compared with CHO (0.087 +/- 0.007 and 0.057 +/- 0.006% 
      h(-1), respectively; P = 0.025). Mitochondrial MPS rates were similar for C+P and 
      CHO (0.082 +/- 0.011 and 0.086 +/- 0.018% h(-1), respectively). mTOR(Ser2448) 
      phosphorylation was greater for C+P compared with CHO at 4 h post-exercise (P < 
      0.05). p70S6K(Thr389) phosphorylation increased at 4 h post-exercise for C+P (P < 
      0.05), whilst eEF2(Thr56) phosphorylation increased by  approximately 40% at 4 h post-exercise 
      for CHO only (P < 0.01). The present study demonstrates that the ingestion of 
      protein in addition to carbohydrate stimulates an increase in myofibrillar, but 
      not mitochondrial, MPS following prolonged cycling. These data indicate that the 
      increase in myofibrillar MPS for C+P could, potentially, be mediated through 
      p70S6K, downstream of mTOR, which in turn may suppress the rise in eEF2 on 
      translation elongation.
FAU - Breen, Leigh
AU  - Breen L
AD  - School of Sport and Exercise Sciences, University of Birmingham, Birmingham, UK. 
      breenl@mcmaster.ca
FAU - Philp, Andrew
AU  - Philp A
FAU - Witard, Oliver C
AU  - Witard OC
FAU - Jackman, Sarah R
AU  - Jackman SR
FAU - Selby, Anna
AU  - Selby A
FAU - Smith, Ken
AU  - Smith K
FAU - Baar, Keith
AU  - Baar K
FAU - Tipton, Kevin D
AU  - Tipton KD
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20110711
PL  - England
TA  - J Physiol
JT  - The Journal of physiology
JID - 0266262
RN  - 0 (Dietary Carbohydrates)
RN  - 0 (Dietary Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Muscle Proteins)
SB  - IM
CIN - J Physiol. 2012 Apr 15;590(Pt 8):1785-6. PMID: 22532648
MH  - Adult
MH  - Beverages
MH  - Cross-Over Studies
MH  - Dietary Carbohydrates/*administration & dosage
MH  - Dietary Proteins/*administration & dosage
MH  - Humans
MH  - Male
MH  - Mitochondrial Proteins/*biosynthesis
MH  - Muscle Proteins/*biosynthesis/physiology
MH  - Myofibrils/*metabolism/physiology
MH  - Physical Endurance/*physiology
MH  - Single-Blind Method
MH  - Young Adult
PMC - PMC3179999
EDAT- 2011/07/13 06:00
MHDA- 2012/05/25 06:00
PMCR- 2012/08/15
CRDT- 2011/07/13 06:00
PHST- 2011/07/13 06:00 [entrez]
PHST- 2011/07/13 06:00 [pubmed]
PHST- 2012/05/25 06:00 [medline]
PHST- 2012/08/15 00:00 [pmc-release]
AID - jphysiol.2011.211888 [pii]
AID - 10.1113/jphysiol.2011.211888 [doi]
PST - ppublish
SO  - J Physiol. 2011 Aug 15;589(Pt 16):4011-25. doi: 10.1113/jphysiol.2011.211888. 
      Epub 2011 Jul 11.