PMID- 21746880
OWN - NLM
STAT- MEDLINE
DCOM- 20111024
LR  - 20231213
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Print)
IS  - 0270-7306 (Linking)
VI  - 31
IP  - 18
DP  - 2011 Sep
TI  - A novel role of vimentin filaments: binding and stabilization of collagen mRNAs.
PG  - 3773-89
LID - 10.1128/MCB.05263-11 [doi]
AB  - The stem-loop in the 5' untranslated region (UTR) of collagen alpha1(I) and alpha2(I) 
      mRNAs (5'SL) is the key element regulating their stability and translation. 
      Stabilization of collagen mRNAs is the predominant mechanism for high collagen 
      expression in fibrosis. LARP6 binds the 5'SL of alpha1(I) and alpha2(I) mRNAs with high 
      affinity. Here, we report that vimentin filaments associate with collagen mRNAs 
      in a 5'SL- and LARP6-dependent manner and stabilize collagen mRNAs. LARP6 
      interacts with vimentin filaments through its La domain and colocalizes with the 
      filaments in vivo. Knockdown of LARP6 by small interfering RNA (siRNA) or 
      mutation of the 5'SL abrogates the interaction of collagen mRNAs with vimentin 
      filaments. Vimentin knockout fibroblasts produce reduced amounts of type I 
      collagen due to decreased stability of collagen alpha1(I) and alpha2(I) mRNAs. Disruption 
      of vimentin filaments using a drug or by expression of dominant-negative desmin 
      reduces type I collagen expression, primarily due to decreased stability of 
      collagen mRNAs. RNA fluorescence in situ hybridization (FISH) experiments show 
      that collagen alpha1(I) and alpha2(I) mRNAs are associated with vimentin filaments in 
      vivo. Thus, vimentin filaments may play a role in the development of tissue 
      fibrosis by stabilizing collagen mRNAs. This finding will serve as a rationale 
      for targeting vimentin in the development of novel antifibrotic therapies.
FAU - Challa, Azariyas A
AU  - Challa AA
AD  - Department of Biomedical Sciences, College of Medicine, Florida State University, 
      Tallahassee, FL 32306, USA.
FAU - Stefanovic, Branko
AU  - Stefanovic B
LA  - eng
GR  - R01 DK059466/DK/NIDDK NIH HHS/United States
GR  - 5R01DK059466-08/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110711
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Autoantigens)
RN  - 0 (Collagen Type I)
RN  - 0 (Desmin)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Ribonucleoproteins)
RN  - 0 (Vimentin)
SB  - IM
MH  - 5' Untranslated Regions
MH  - Animals
MH  - Autoantigens/genetics/*metabolism
MH  - Cell Line
MH  - Collagen Type I/biosynthesis/*genetics/metabolism
MH  - Desmin/biosynthesis
MH  - Fibroblasts/metabolism
MH  - Fibrosis/genetics/metabolism
MH  - Gene Knock-In Techniques
MH  - Gene Knockout Techniques
MH  - HEK293 Cells
MH  - Humans
MH  - In Situ Hybridization
MH  - Intermediate Filaments/*metabolism
MH  - Inverted Repeat Sequences
MH  - Mice
MH  - Mice, Knockout
MH  - Nucleic Acid Conformation
MH  - Polymerase Chain Reaction
MH  - RNA Interference
MH  - RNA, Messenger/*genetics/*metabolism
MH  - RNA, Small Interfering
MH  - RNA-Binding Proteins/genetics/metabolism
MH  - Ribonucleoproteins/genetics/*metabolism
MH  - Vimentin/genetics/*metabolism
MH  - SS-B Antigen
PMC - PMC3165730
EDAT- 2011/07/13 06:00
MHDA- 2011/10/25 06:00
PMCR- 2012/03/01
CRDT- 2011/07/13 06:00
PHST- 2011/07/13 06:00 [entrez]
PHST- 2011/07/13 06:00 [pubmed]
PHST- 2011/10/25 06:00 [medline]
PHST- 2012/03/01 00:00 [pmc-release]
AID - MCB.05263-11 [pii]
AID - 5263-11 [pii]
AID - 10.1128/MCB.05263-11 [doi]
PST - ppublish
SO  - Mol Cell Biol. 2011 Sep;31(18):3773-89. doi: 10.1128/MCB.05263-11. Epub 2011 Jul 
      11.