PMID- 21747336
OWN - NLM
STAT- MEDLINE
DCOM- 20111123
LR  - 20211203
IS  - 1559-0488 (Electronic)
IS  - 1559-047X (Linking)
VI  - 32
IP  - 4
DP  - 2011 Jul-Aug
TI  - Culture medium and cell density impact gene expression in normal skin and 
      abnormal scar-derived fibroblasts.
PG  - 498-508
LID - 10.1097/BCR.0b013e3182223cb1 [doi]
AB  - Fibroblasts, the main cell type of the dermis, are responsible for production and 
      remodeling of extracellular matrix during wound healing. Disruption of either 
      production or degradation of extracellular matrix can lead to abnormal scarring, 
      resulting in hypertrophic scar or keloid scar. Aberrations in proliferation and 
      gene expression have been observed in fibroblasts isolated from abnormal scars, 
      but differences observed may be related to biologic responses to growth 
      conditions and media formulations. This study examined gene expression in primary 
      human fibroblasts from normal skin or abnormal scar in two culture media 
      formulations and three relative cell densities. In general, higher expression of 
      collagen type 1 alpha-1 (COL1A1) and alpha-2 (COL1A2) and matrix 
      metalloproteinase 3 (MMP3) and lower levels of MMP1 were observed in all cell 
      strains cultured in standard medium containing 10% fetal bovine serum compared 
      with cells cultured in medium optimized for proliferation. Normal and 
      scar-derived fibroblasts exhibited differences in gene expression in specific 
      response to media formulations and cell density. COL1A1 and COL1A2 were 
      increased, and MMP1 and MMP3 were decreased, in keloid cells compared with normal 
      fibroblasts under most conditions analyzed. However, expression of plasminogen 
      activator inhibitor 1 in keloid fibroblasts, which was significantly different 
      than in normal fibroblasts, was either increased or decreased in response to the 
      medium formulation and relative cell density. A related gene, plasminogen 
      activator inhibitor 2, was shown for the first time to be significantly increased 
      in keloid fibroblasts compared with normal fibroblasts, in both media 
      formulations and at all three cell densities. The results emphasize the critical 
      role of culture conditions in interpretation of cell behavior and expression data 
      and for comparison of cells representing normal and fibrotic phenotypes.
FAU - McFarland, Kevin L
AU  - McFarland KL
AD  - Research Department, Shriners Hospitals for Children, Cincinnati, Ohio 45229, 
      USA.
FAU - Glaser, Kathryn
AU  - Glaser K
FAU - Hahn, Jennifer M
AU  - Hahn JM
FAU - Boyce, Steven T
AU  - Boyce ST
FAU - Supp, Dorothy M
AU  - Supp DM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Burn Care Res
JT  - Journal of burn care & research : official publication of the American Burn 
      Association
JID - 101262774
RN  - 0 (Collagen Type I)
RN  - 0 (Collagen Type I, alpha 1 Chain)
RN  - 0 (Culture Media)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.7 (Matrix Metalloproteinase 1)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Cell Proliferation/*drug effects
MH  - Cells, Cultured
MH  - Cicatrix, Hypertrophic/*metabolism/pathology
MH  - Collagen Type I/*metabolism
MH  - Collagen Type I, alpha 1 Chain
MH  - Culture Media/pharmacology
MH  - Fibroblasts/drug effects/*metabolism
MH  - Gene Expression Regulation/*drug effects
MH  - Humans
MH  - Matrix Metalloproteinase 1/metabolism
MH  - Matrix Metalloproteinase 3/metabolism
MH  - Serum/metabolism
EDAT- 2011/07/13 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/07/13 06:00
PHST- 2011/07/13 06:00 [entrez]
PHST- 2011/07/13 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 01253092-201107000-00007 [pii]
AID - 10.1097/BCR.0b013e3182223cb1 [doi]
PST - ppublish
SO  - J Burn Care Res. 2011 Jul-Aug;32(4):498-508. doi: 10.1097/BCR.0b013e3182223cb1.