PMID- 21748319
OWN - NLM
STAT- MEDLINE
DCOM- 20120628
LR  - 20211020
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 39
IP  - 4
DP  - 2012 Apr
TI  - Molecular analysis of a ras-like nuclear (Ran) gene from Penaeus monodon and its 
      expression at the different ovarian stages of development.
PG  - 3821-7
LID - 10.1007/s11033-011-1160-0 [doi]
AB  - In the present study, a ras-like nuclear (Ran) gene was obtained from the ovary 
      and neurosecretory organ in eyestalk cDNA library of black tiger prawn (Penaeus 
      monodon). The full-length black tiger prawn Ran (PmRan) cDNA consisted of 1140 
      nucleotides including an open reading frame (ORF) 648 bp, a 5' untranslated 
      region (5'UTR) of 117 bp and a 3'UTR of 375 bp with a polyadenylation signal 
      sequence "aataaa" and a poly (A) tail. The ORF encoded a peptide of 215 amino 
      acids with molecular mass 24.6 kDa and a theoretical isoelectric point of 7.39. 
      ScanProsite analysis indicated that PmRan protein sequence contained a small 
      GTPase Ran family motif. Homology analysis of the deduced amino acid sequence of 
      the PmRan with other known Ran sequences by MatGAT software revealed that the 
      PmRan show very high homology with the sequences of other animals (92.1-98.6% 
      similarity, 85.6-98.1% identity). Analysis of the tissue expression pattern of 
      the PmRan gene showed that the PmRan mRNA was expressed in all tested tissues, 
      including hepatopancreas, ovary, muscle, intestine, neurosecretory organ in 
      eyestalk, neurosecretory organ in brain, stomach, and heart, with the highest 
      levels in ovary. Furthermore, the PmRan expression was found to be high level in 
      the six ovarian stages of development. The results indicated PmRan might play an 
      important role in ovarian development.
FAU - Zhou, Falin
AU  - Zhou F
AD  - South China Sea Fisheries Research Institute, CAFS, 231 Xingang Road Western, 
      Guangzhou, 510300, Guangdong, China.
FAU - Zheng, Liming
AU  - Zheng L
FAU - Yang, Qibin
AU  - Yang Q
FAU - Qiu, Lihua
AU  - Qiu L
FAU - Huang, Jianhua
AU  - Huang J
FAU - Su, Tiannfeng
AU  - Su T
FAU - Jiang, Shigui
AU  - Jiang S
LA  - eng
SI  - GENBANK/HQ647123
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110712
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.5.2 (ran GTP-Binding Protein)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - Female
MH  - *Gene Expression Regulation, Developmental
MH  - Molecular Sequence Data
MH  - Ovary/cytology/*growth & development/*metabolism
MH  - Penaeidae/cytology/*genetics/*growth & development
MH  - Phylogeny
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Sequence Homology, Amino Acid
MH  - ran GTP-Binding Protein/*genetics/metabolism
EDAT- 2011/07/13 06:00
MHDA- 2012/06/29 06:00
CRDT- 2011/07/13 06:00
PHST- 2011/02/14 00:00 [received]
PHST- 2011/06/30 00:00 [accepted]
PHST- 2011/07/13 06:00 [entrez]
PHST- 2011/07/13 06:00 [pubmed]
PHST- 2012/06/29 06:00 [medline]
AID - 10.1007/s11033-011-1160-0 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2012 Apr;39(4):3821-7. doi: 10.1007/s11033-011-1160-0. Epub 2011 
      Jul 12.