PMID- 21749409
OWN - NLM
STAT- MEDLINE
DCOM- 20111101
LR  - 20131121
IS  - 1525-1594 (Electronic)
IS  - 0160-564X (Linking)
VI  - 35
IP  - 7
DP  - 2011 Jul
TI  - Improved neuronal survival of focal ischemia after delipid extracorporeal 
      lipoprotein treatment in hyperlipidemic rabbits.
PG  - E145-54
LID - 10.1111/j.1525-1594.2011.01249.x [doi]
AB  - A delipid extracorporeal lipoprotein filter (DELP) system has been used to treat 
      patients with stroke and has shown favorable prognosis. However, the mechanism 
      for the neuronal functional recovery remains unclear. This study aimed to 
      investigate the neuronal histological assessment, and the levels of C-reactive 
      protein (CRP), tumor necrosis factor alpha (TNF-alpha), and oxidized low-density 
      lipoprotein (oxLDL) after ischemic stroke following DELP treatment. 
      Hyperlipidemic rabbits underwent middle cerebral artery occlusion. After 30 min, 
      the animals received an extracorporeal apheresis treatment with a DELP filter. 
      Total cholesterol (TC), triglyceride, and low-density lipoprotein (LDL) of the 
      plasma were measured. The levels of CRP, TNF-alpha, and oxLDL in brain tissue were 
      also measured by enzyme-linked immunosorbent assay. Hematoxylin and eosin 
      staining, cresyl violet staining, neuron-specific enolase (NSE) and glial 
      fibrillary acidic protein immunohistochemistry, and terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) assay were performed. DELP 
      apheresis reduced TC and LDL by >30%. The number of neurons at day 7 (P < 0.01) 
      and the integrated optical density of NSE at day 1 (P < 0.05) and day 7 (P < 
      0.01) were significantly increased in the DELP group. TUNEL-positive cells were 
      significantly decreased (P < 0.05). Astrocytes were moderately activated, and 
      this activation persisted up to 7 days. Gliosis was not found in the DELP group. 
      After treatment, the level of CRP declined at day 1 (P < 0.05); TNF-alpha and oxLDL 
      declined at day 7 (P < 0.05). DELP apheresis decreased neuronal apoptosis, 
      reduced inflammatory markers and oxidative stress in cerebral ischemia, and 
      improved neuronal survival.
CI  - (c) 2011, Copyright the Authors. Artificial Organs (c) 2011, International Center for 
      Artificial Organs and Transplantation and Wiley Periodicals, Inc.
FAU - Liu, Guojing
AU  - Liu G
AD  - Medical School of Nankai University, Tianjin, China.
FAU - Wang, Weichao
AU  - Wang W
FAU - Xie, Hui
AU  - Xie H
FAU - Che, Yongzhe
AU  - Che Y
FAU - Xue, Jun
AU  - Xue J
FAU - Wu, Youzhen
AU  - Wu Y
FAU - Zhou, Qingwen
AU  - Zhou Q
FAU - Kong, Deling
AU  - Kong D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Artif Organs
JT  - Artificial organs
JID - 7802778
RN  - 0 (Lipoproteins)
RN  - 0 (Lipoproteins, LDL)
RN  - 0 (Triglycerides)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (oxidized low density lipoprotein)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 97C5T2UQ7J (Cholesterol)
SB  - IM
MH  - Animals
MH  - Blood Component Removal/*instrumentation
MH  - Brain/metabolism/pathology
MH  - Brain Ischemia/blood/*metabolism/pathology/*therapy
MH  - C-Reactive Protein/metabolism
MH  - Cell Survival
MH  - Cholesterol/blood
MH  - Equipment Design
MH  - Hyperlipidemias/blood/metabolism/pathology/*therapy
MH  - Lipoproteins/blood/*isolation & purification/metabolism
MH  - Lipoproteins, LDL/blood/isolation & purification/metabolism
MH  - Male
MH  - Neurons/cytology/metabolism/*pathology
MH  - Rabbits
MH  - Triglycerides/blood
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2011/07/14 06:00
MHDA- 2011/11/02 06:00
CRDT- 2011/07/14 06:00
PHST- 2011/07/14 06:00 [entrez]
PHST- 2011/07/14 06:00 [pubmed]
PHST- 2011/11/02 06:00 [medline]
AID - 10.1111/j.1525-1594.2011.01249.x [doi]
PST - ppublish
SO  - Artif Organs. 2011 Jul;35(7):E145-54. doi: 10.1111/j.1525-1594.2011.01249.x.