PMID- 21750540
OWN - NLM
STAT- MEDLINE
DCOM- 20111018
LR  - 20230815
IS  - 2041-1723 (Electronic)
IS  - 2041-1723 (Linking)
VI  - 2
DP  - 2011 Jul 12
TI  - A photoconvertible fluorescent reporter to track chaperone-mediated autophagy.
PG  - 386
LID - 10.1038/ncomms1393 [doi]
AB  - Chaperone-mediated autophagy (CMA) is a selective mechanism for the degradation 
      of soluble proteins in lysosomes. CMA contributes to cellular quality control and 
      is activated as part of the cellular response to different stressors. Defective 
      CMA has been identified in ageing and different age-related diseases. Until now, 
      CMA activity could only be measured in vitro using isolated lysosomes. Here we 
      report the development of a photoconvertible fluorescent reporter that allows 
      monitoring of CMA activity in living cells. Activation of CMA increases the 
      association of the reporter with lysosomes which can be visualized as a change in 
      the intracellular fluorescence. The CMA reporter can be utilized in a broad 
      variety of cells and is suitable for high-content microscopy. Using this 
      reporter, we find that levels of basal and inducible CMA activity are cell-type 
      dependent, and we have identified an upregulation of this pathway in response to 
      the catalytic inhibition of the proteasome.
FAU - Koga, Hiroshi
AU  - Koga H
AD  - Department of Developmental and Molecular Biology, Albert Einstein College of 
      Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA.
FAU - Martinez-Vicente, Marta
AU  - Martinez-Vicente M
FAU - Macian, Fernando
AU  - Macian F
FAU - Verkhusha, Vladislav V
AU  - Verkhusha VV
FAU - Cuervo, Ana Maria
AU  - Cuervo AM
LA  - eng
GR  - R01 AG021904/AG/NIA NIH HHS/United States
GR  - AG031782/AG/NIA NIH HHS/United States
GR  - R01 GM073913/GM/NIGMS NIH HHS/United States
GR  - GM073913/GM/NIGMS NIH HHS/United States
GR  - R37 AG021904/AG/NIA NIH HHS/United States
GR  - P01 AG031782/AG/NIA NIH HHS/United States
GR  - AG021904/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110712
PL  - England
TA  - Nat Commun
JT  - Nature communications
JID - 101528555
RN  - 0 (Molecular Chaperones)
SB  - IM
MH  - Analysis of Variance
MH  - Animals
MH  - Autophagy/*physiology
MH  - Cell Line, Tumor
MH  - Flow Cytometry
MH  - Fluorescence
MH  - Humans
MH  - Lysosomes/*physiology
MH  - Mice
MH  - Molecular Chaperones/metabolism/*physiology
MH  - *Molecular Probe Techniques
MH  - NIH 3T3 Cells
MH  - Plasmids/genetics
PMC - PMC3529934
MID - NIHMS425440
COIS- Competing interest. The authors declare no competing financial interest.
EDAT- 2011/07/14 06:00
MHDA- 2011/10/19 06:00
PMCR- 2012/12/25
CRDT- 2011/07/14 06:00
PHST- 2011/01/05 00:00 [received]
PHST- 2011/06/15 00:00 [accepted]
PHST- 2011/07/14 06:00 [entrez]
PHST- 2011/07/14 06:00 [pubmed]
PHST- 2011/10/19 06:00 [medline]
PHST- 2012/12/25 00:00 [pmc-release]
AID - ncomms1393 [pii]
AID - 10.1038/ncomms1393 [doi]
PST - epublish
SO  - Nat Commun. 2011 Jul 12;2:386. doi: 10.1038/ncomms1393.