PMID- 21751868
OWN - NLM
STAT- MEDLINE
DCOM- 20111118
LR  - 20220316
IS  - 1480-3321 (Electronic)
IS  - 0831-2796 (Linking)
VI  - 54
IP  - 7
DP  - 2011 Jul
TI  - Painting the rye genome with genome-specific sequences.
PG  - 555-64
AB  - We used rye-specific repetitive DNA sequences in fluorescence in situ 
      hybridization (FISH) to paint the rye genome and to identify rye DNA in a wheat 
      background. A 592 bp fragment from the rye-specific dispersed repetitive family 
      R173 (named UCM600) was cloned and used as a FISH probe. UCM600 is dispersed over 
      the seven rye chromosomes, being absent from the pericentromeric and subtelomeric 
      regions. A similar pattern of distribution was also observed on the rye B 
      chromosomes, but with weaker signals. The FISH hybridization patterns using 
      UCM600 as probe were comparable with those obtained with the genomic in situ 
      hybridization (GISH) procedure. There were, however, sharper signals and less 
      background with FISH. UCM600 was combined with the rye-specific sequences Bilby 
      and pSc200 to obtain a more complete painting. With these probes, the rye 
      chromosomes were labeled with distinctive patterns; thus, allowing the rye 
      cultivar 'Imperial' to be karyotyped. It was also possible to distinguish rye 
      chromosomes in triticale and alien rye chromatin in wheat-rye addition and 
      translocation lines. The distribution of UCM600 was similar in cultivated rye and 
      in the wild Secale species Secale vavilovii Grossh., Secale sylvestre Host, and 
      Secale africanum Stapf. Thus, UCM600 can be used to detect Secale DNA 
      introgressed from wild species in a wheat background.
FAU - Gonzalez-Garcia, Miriam
AU  - Gonzalez-Garcia M
AD  - Departamento de Genetica, Universidad Complutense, Madrid, Spain.
FAU - Cuacos, Maria
AU  - Cuacos M
FAU - Gonzalez-Sanchez, Monica
AU  - Gonzalez-Sanchez M
FAU - Puertas, Maria J
AU  - Puertas MJ
FAU - Vega, Juan M
AU  - Vega JM
LA  - eng
SI  - GENBANK/EU543274
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110713
PL  - Canada
TA  - Genome
JT  - Genome
JID - 8704544
SB  - IM
MH  - Base Sequence
MH  - Chromosomes, Plant/genetics
MH  - Cloning, Molecular
MH  - Genes, Plant
MH  - Genome, Plant/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Molecular Sequence Data
MH  - Secale/*genetics
MH  - Translocation, Genetic
EDAT- 2011/07/15 06:00
MHDA- 2011/12/13 00:00
CRDT- 2011/07/15 06:00
PHST- 2011/07/15 06:00 [entrez]
PHST- 2011/07/15 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
AID - 10.1139/g11-003 [doi]
PST - ppublish
SO  - Genome. 2011 Jul;54(7):555-64. doi: 10.1139/g11-003. Epub 2011 Jul 13.