PMID- 21755524
OWN - NLM
STAT- MEDLINE
DCOM- 20120130
LR  - 20171116
IS  - 1099-0844 (Electronic)
IS  - 0263-6484 (Linking)
VI  - 29
IP  - 7
DP  - 2011 Oct
TI  - Ex vivo stimulation of murine dendritic cells by an exopolysaccharide from one of 
      the anamorph of Cordyceps sinensis.
PG  - 555-61
LID - 10.1002/cbf.1787 [doi]
AB  - Dendritic cells (DCs) play an important role in initiating antitumour immune 
      response. Tumour progression usually induces defects in DC maturation and thus 
      tumour-bearing hosts exhibit immunosuppression and tumour escape. The previous 
      studies showed that an exopolysaccharide (EPS) from a fungus, one anamorph of 
      Cordyceps sinensis, inhibited tumour growth via activating immune response in the 
      hosts. In view of the crucial actions of DCs in antitumour immunity, the present 
      study aims to explore the effects of EPS on murine DCs. Murine DCs were derived 
      from the bone marrow of C57BL/6 mice, and the effects of EPS on phenotype 
      molecules and ingestion function of DCs were assayed using flow cytometry. 
      Cytokine expressions of DCs were assayed by reverse transcriptase-PCR. 
      Additionally, the level of phosphorylated signal transducers and activators of 
      transcription 3 (p-STAT3) of DCs was evaluated using Western blotting. The 
      results showed that EPS promoted the levels of surface molecules MHC II, CD40, 
      CD80 and CD86 of DCs and decreased their ingestion ability. The mRNA expressions 
      of cytokines (IL-12p40 and TNF-alpha) and inducible nitric oxide synthase were 
      up-regulated by EPS. We also found that EPS significantly down-regulated p-STAT3 
      level of DCs. The results suggested that the promotion of DC's maturation and 
      activation by EPS is probably related to the inhibition of STAT3 phosphorylation.
CI  - Copyright (c) 2011 John Wiley & Sons, Ltd.
FAU - Song, Dan
AU  - Song D
AD  - Jiangsu Key Laboratory of Molecular Medicine, Medical School and State Key 
      Laboratory of Analytical Chemistry for Life Science, Nanjing University, Nanjing, 
      China.
FAU - Lin, Jianyu
AU  - Lin J
FAU - Yuan, Fengjiao
AU  - Yuan F
FAU - Zhang, Weiyun
AU  - Zhang W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110714
PL  - England
TA  - Cell Biochem Funct
JT  - Cell biochemistry and function
JID - 8305874
RN  - 0 (B7-2 Antigen)
RN  - 0 (CD40 Antigens)
RN  - 0 (Cd86 protein, mouse)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Interleukin-12 Subunit p40)
RN  - 0 (Polysaccharides)
RN  - 0 (RNA, Messenger)
RN  - 0 (STAT3 Transcription Factor)
RN  - 0 (Stat3 protein, mouse)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - EC 1.14.13.39 (Nos2 protein, mouse)
SB  - IM
MH  - Animals
MH  - B7-2 Antigen/immunology/metabolism
MH  - Bone Marrow Cells/cytology/immunology
MH  - CD40 Antigens/immunology/metabolism
MH  - Cell Differentiation
MH  - Cordyceps/chemistry/*immunology
MH  - Dendritic Cells/cytology/drug effects/*immunology
MH  - Endocytosis
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression Regulation
MH  - Histocompatibility Antigens Class II/immunology/metabolism
MH  - Interleukin-12 Subunit p40/immunology/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Nitric Oxide Synthase Type II/immunology/metabolism
MH  - Phenotype
MH  - Polysaccharides/immunology/*pharmacology
MH  - RNA, Messenger/analysis/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - STAT3 Transcription Factor/genetics/metabolism
MH  - Signal Transduction
MH  - Transcriptional Activation
MH  - Tumor Necrosis Factor-alpha/immunology/metabolism
EDAT- 2011/07/15 06:00
MHDA- 2012/01/31 06:00
CRDT- 2011/07/15 06:00
PHST- 2011/01/10 00:00 [received]
PHST- 2011/04/19 00:00 [revised]
PHST- 2011/06/06 00:00 [accepted]
PHST- 2011/07/15 06:00 [entrez]
PHST- 2011/07/15 06:00 [pubmed]
PHST- 2012/01/31 06:00 [medline]
AID - 10.1002/cbf.1787 [doi]
PST - ppublish
SO  - Cell Biochem Funct. 2011 Oct;29(7):555-61. doi: 10.1002/cbf.1787. Epub 2011 Jul 
      14.