PMID- 2175590
OWN - NLM
STAT- MEDLINE
DCOM- 19910128
LR  - 20190815
IS  - 0304-8608 (Print)
IS  - 0304-8608 (Linking)
VI  - 115
IP  - 3-4
DP  - 1990
TI  - Establishment of transformed swine fibroblast cell lines using SV40 large T 
      antigen.
PG  - 227-37
AB  - Swine testicle cell lines were established by transformation of primary swine 
      testicle (PST) cells with an SV40 plasmid (pSV3-neo), which contains genes 
      conferring resistance to neomycin and expressing SV40 large T antigen. Plasmid 
      DNA was transfected into PST cells using a lipofection system. Two related 
      plasmids, pSV2-neo and pSV5-neo, failed to induce transformed cells. Cells 
      transformed with pSV3-neo formed single colonies that were resistant to the 
      antibiotic, G418, and expressed large T antigen. Upon two cycles of cloning by 
      endpoint dilution method, three transformed clones, designated transformed swine 
      testicle (tST)-3, tST-14 and tST-18, were selected and characterized in regards 
      to cell replication and susceptibility to swine viruses. The resultant clones 
      were compared with a counterpart non-transformed ST cell line (ATCC-ST). The 
      three tST cell lines showed longer or the same doubling times and higher 
      saturation densities compared to ATCC-ST cells. These cells were free from a 
      range of adventitious agents and supported the replication of porcine parvovirus 
      (PPV), pseudorabies virus (PRV) and transmissible gastroenteritis virus (TGEV), 
      comparable to ATCC-ST cells. All three cell lines have been maintained in 
      continuous cultures for over 60 passages with no changes in growth 
      characteristics. These findings indicate that lipofection with pSV3-neo is an 
      efficient means for the introduction of exogenous DNA into porcine cells and for 
      establishment of transformed immortalized cell lines.
FAU - Choi, C S
AU  - Choi CS
AD  - Department of Large Animal Clinical Sciences, College of Veterinary Medicine, 
      University of Minnesota, St. Paul.
FAU - Murtaugh, M P
AU  - Murtaugh MP
FAU - Molitor, T W
AU  - Molitor TW
LA  - eng
PT  - Journal Article
PL  - Austria
TA  - Arch Virol
JT  - Archives of virology
JID - 7506870
RN  - 0 (Antigens, Polyomavirus Transforming)
SB  - IM
MH  - Animals
MH  - *Antigens, Polyomavirus Transforming
MH  - Cell Division
MH  - *Cell Line, Transformed
MH  - *Cell Transformation, Viral
MH  - Clone Cells
MH  - Fibroblasts
MH  - Male
MH  - Plasmids
MH  - Simian virus 40
MH  - Swine
MH  - Testis/cytology
MH  - Transfection
MH  - Viruses/isolation & purification
EDAT- 1990/01/01 00:00
MHDA- 1990/01/01 00:01
CRDT- 1990/01/01 00:00
PHST- 1990/01/01 00:00 [pubmed]
PHST- 1990/01/01 00:01 [medline]
PHST- 1990/01/01 00:00 [entrez]
AID - 10.1007/BF01310532 [doi]
PST - ppublish
SO  - Arch Virol. 1990;115(3-4):227-37. doi: 10.1007/BF01310532.