PMID- 21756194
OWN - NLM
STAT- MEDLINE
DCOM- 20120606
LR  - 20220912
IS  - 1478-6427 (Electronic)
IS  - 1478-6419 (Linking)
VI  - 26
IP  - 6
DP  - 2012
TI  - LC determination of luteolin-7-O-beta-D-glucoside and apigenin-7-O-beta-D-glucoside in 
      rat plasma after administration of Humulus scandens extract and its application 
      to pharmacokinetic studies.
PG  - 530-9
LID - 10.1080/14786419.2010.532127 [doi]
AB  - The present study was to investigate the pharmacokinetics of 
      luteolin-7-O-beta-D-glucoside (LGL) and apigenin-7-O-beta-D-glucoside (AGL) in rat 
      plasma after intravenous administration of the Humulus scandens extract (HSE). A 
      simple and accurate high-performance liquid chromatographic (HPLC) method was 
      successfully developed for simultaneous determination of LGL and AGL in rat 
      plasma after the plasma protein was precipitated with methanol. HPLC analysis was 
      performed on a C(1)(8) column with UV detection at 350 nm and a mobile phase of 
      methanol-0.2% phosphoric acid (1 : 1, v/v). Calibration curves of LGL and AGL 
      were linear over the concentration range of 0.16-20.0 and 0.06-7.20 microg mL(-)(1), 
      respectively. The accuracy and precision of the two analytes at low, medium and 
      high concentrations were within the range of -3.4% to 8.1%. The relative standard 
      deviations (RSDs) of the intra- and inter-day precisions were less than 11.7% and 
      10.0%, respectively. The extraction recoveries (n = 5) varied from 91.9% to 
      104.1% for LGL and from 92.6% to 109.3% for AGL. The method was fully validated 
      and successfully applied to a pharmacokinetic study of LGL and AGL in rat plasma 
      after the intravenous administration of HSE.
FAU - Chen, Zaixing
AU  - Chen Z
AD  - School of Pharmaceutical Sciences, China Medical University, 92 Beier Road, 
      Shenyang, Liaoning Province 110001, People's Republic of China.
FAU - Ying, Xixiang
AU  - Ying X
FAU - Meng, Shu
AU  - Meng S
FAU - Zhu, Xu
AU  - Zhu X
FAU - Jiang, Hong
AU  - Jiang H
FAU - Cao, Qishen
AU  - Cao Q
FAU - Wang, Lin
AU  - Wang L
FAU - Meng, Fanhao
AU  - Meng F
LA  - eng
PT  - Journal Article
DEP - 20110715
PL  - England
TA  - Nat Prod Res
JT  - Natural product research
JID - 101167924
RN  - 0 (Flavones)
RN  - 0 (Glucosides)
RN  - 0 (Plant Extracts)
RN  - 7OF2S66PCH (apigetrin)
RN  - 7V515PI7F6 (Apigenin)
RN  - 98J6XDS46I (luteolin-7-glucoside)
SB  - IM
MH  - Animals
MH  - Apigenin/*blood
MH  - Calibration
MH  - Chromatography, High Pressure Liquid
MH  - Flavones/*blood
MH  - Glucosides/*blood
MH  - Humulus/*chemistry
MH  - Injections, Intravenous
MH  - Plant Extracts/administration & dosage/*pharmacokinetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Reproducibility of Results
EDAT- 2011/07/16 06:00
MHDA- 2012/06/07 06:00
CRDT- 2011/07/16 06:00
PHST- 2011/07/16 06:00 [entrez]
PHST- 2011/07/16 06:00 [pubmed]
PHST- 2012/06/07 06:00 [medline]
AID - 10.1080/14786419.2010.532127 [doi]
PST - ppublish
SO  - Nat Prod Res. 2012;26(6):530-9. doi: 10.1080/14786419.2010.532127. Epub 2011 Jul 
      15.