PMID- 21756254
OWN - NLM
STAT- MEDLINE
DCOM- 20120105
LR  - 20131121
IS  - 1601-5223 (Electronic)
IS  - 0018-0661 (Linking)
VI  - 148
IP  - 3
DP  - 2011 Jun
TI  - Exclusion of Sall 4 as the sex-determining gene in the Mandarin vole Microtus 
      mandarinus mandarinus.
PG  - 93-7
LID - 10.1111/j.1601-5223.2011.02207.x [doi]
AB  - In previous studies, we have shown that the Sry HMG-box is absent in Microtus 
      mandarinus mandarinus (M. m. mandarinus), suggesting that sex determination of M. 
      m. mandarinus is independent of the Sry gene. We amplified a 312 bp fragment 
      within exon 2 of the Sall4 gene in the mouse and M. m. mandarinus using 
      polymerase chain reaction (PCR) and detected Sall4 using fluorescence in situ 
      hybridization (FISH) technique. The probes for the Sall4 gene were labeled with 
      digoxigenin using PCR and hybridized to chromosomes and interphase nuclei of the 
      mouse and M. m. mandarinus. Our results suggested that Sall4 exists in the genome 
      of male and female M. m. mandarinus, and the sequence within exon 2 of the gene 
      is the same in the mouse and M. m. mandarinus. The results also showed that Sall4 
      is localized on chromosome 6 in M. m. mandarinus. As they are the sex chromosomes 
      in M. m. mandarinus, the results excluded the Sall4 gene from being the 
      testis-determining factor in this species. We propose that in M. m. mandarinus, 
      sex determination is controlled by another yet unknown gene on the sex 
      chromosomes.
CI  - (c) 2011 The Authors.
FAU - Chen, Yanqiu
AU  - Chen Y
AD  - School of Life Science, Xuhou Normal University, Jiangsu, PR China.
FAU - Ming, Qinglei
AU  - Ming Q
FAU - Zhu, Bicai
AU  - Zhu B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110615
PL  - England
TA  - Hereditas
JT  - Hereditas
JID - 0374654
RN  - 0 (DNA Probes)
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Sall4 protein, mouse)
RN  - 0 (Transcription Factors)
RN  - NQ1SX9LNAU (Digoxigenin)
SB  - IM
MH  - Animals
MH  - Arvicolinae/*genetics
MH  - Chromosome Mapping
MH  - Chromosomes, Mammalian/genetics
MH  - DNA Probes/chemistry/genetics
MH  - DNA-Binding Proteins/*genetics
MH  - Digoxigenin/chemistry
MH  - Female
MH  - In Situ Hybridization, Fluorescence
MH  - Male
MH  - Mice
MH  - Sex Chromosomes/*genetics
MH  - Sex Determination Processes
MH  - Transcription Factors/*genetics
EDAT- 2011/07/16 06:00
MHDA- 2012/01/06 06:00
CRDT- 2011/07/16 06:00
PHST- 2011/07/16 06:00 [entrez]
PHST- 2011/07/16 06:00 [pubmed]
PHST- 2012/01/06 06:00 [medline]
AID - 10.1111/j.1601-5223.2011.02207.x [doi]
PST - ppublish
SO  - Hereditas. 2011 Jun;148(3):93-7. doi: 10.1111/j.1601-5223.2011.02207.x. Epub 2011 
      Jun 15.