PMID- 21757229
OWN - NLM
STAT- MEDLINE
DCOM- 20111212
LR  - 20220316
IS  - 1878-5905 (Electronic)
IS  - 0142-9612 (Print)
IS  - 0142-9612 (Linking)
VI  - 32
IP  - 30
DP  - 2011 Oct
TI  - The fast release of stem cells from alginate-fibrin microbeads in injectable 
      scaffolds for bone tissue engineering.
PG  - 7503-13
LID - 10.1016/j.biomaterials.2011.06.045 [doi]
AB  - Stem cell-encapsulating hydrogel microbeads of several hundred microns in size 
      suitable for injection, that could quickly degrade to release the cells, are 
      currently unavailable. The objectives of this study were to: (1) develop oxidized 
      alginate-fibrin microbeads encapsulating human umbilical cord mesenchymal stem 
      cells (hUCMSCs); (2) investigate microbead degradation, cell release, and 
      osteogenic differentiation of the released cells for the first time. Three types 
      of microbeads were fabricated to encapsulate hUCMSCs: (1) Alginate microbeads; 
      (2) oxidized alginate microbeads; (3) oxidized alginate-fibrin microbeads. 
      Microbeads with sizes of about 100-500 mum were fabricated with 1 x 10(6) 
      hUCMSCs/mL of alginate. For the alginate group, there was little microbead 
      degradation, with very few cells released at 21 d. For oxidized alginate, the 
      microbeads started to slightly degrade at 14 d. In contrast, the oxidized 
      alginate-fibrin microbeads started to degrade at 4 d and released the cells. At 7 
      d, the number of released cells greatly increased and showed a healthy polygonal 
      morphology. At 21 d, the oxidized alginate-fibrin group had a live cell density 
      that was 4-fold that of the oxidized alginate group, and 15-fold that of the 
      alginate group. The released cells had osteodifferentiation, exhibiting highly 
      elevated bone marker gene expressions of ALP, OC, collagen I, and Runx2. Alizarin 
      staining confirmed the synthesis of bone minerals by hUCMSCs, with the mineral 
      concentration at 21 d being 10-fold that at 7 d. In conclusion, fast-degradable 
      alginate-fibrin microbeads with hUCMSC encapsulation were developed that could 
      start to degrade and release the cells at 4 d. The released hUCMSCs had excellent 
      proliferation, osteodifferentiation, and bone mineral synthesis. The 
      alginate-fibrin microbeads are promising to deliver stem cells inside injectable 
      scaffolds to promote tissue regeneration.
CI  - Published by Elsevier Ltd.
FAU - Zhou, Hongzhi
AU  - Zhou H
AD  - Biomaterials & Tissue Engineering Division, Department of Endodontics, 
      Prosthodontics and Operative Dentistry, University of Maryland Dental School, 
      Baltimore, MD 21201, USA.
FAU - Xu, Hockin H K
AU  - Xu HH
LA  - eng
GR  - R01 DE014190-09/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-07A1S1/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190/DE/NIDCR NIH HHS/United States
GR  - R01 DE14190/DE/NIDCR NIH HHS/United States
GR  - R01 DE17974/DE/NIDCR NIH HHS/United States
GR  - R01 DE017974/DE/NIDCR NIH HHS/United States
GR  - R01 DE014190-08/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110714
PL  - Netherlands
TA  - Biomaterials
JT  - Biomaterials
JID - 8100316
RN  - 0 (Alginates)
RN  - 0 (Hexuronic Acids)
RN  - 25852-47-5 (Hydrogel, Polyethylene Glycol Dimethacrylate)
RN  - 8A5D83Q4RW (Glucuronic Acid)
RN  - 9001-31-4 (Fibrin)
SB  - IM
MH  - Alginates/*chemistry
MH  - Bone and Bones/*cytology
MH  - Cell Differentiation
MH  - Cells, Cultured
MH  - Fibrin/*chemistry
MH  - Glucuronic Acid/chemistry
MH  - Hexuronic Acids/chemistry
MH  - Humans
MH  - Hydrogel, Polyethylene Glycol Dimethacrylate/chemistry
MH  - Mesenchymal Stem Cells/*cytology
MH  - Microspheres
MH  - Osteogenesis
MH  - Tissue Engineering/*methods
MH  - Tissue Scaffolds/*chemistry
MH  - Umbilical Cord/cytology
PMC - PMC3159408
MID - NIHMS308519
EDAT- 2011/07/16 06:00
MHDA- 2011/12/14 06:00
PMCR- 2012/10/01
CRDT- 2011/07/16 06:00
PHST- 2011/05/03 00:00 [received]
PHST- 2011/06/19 00:00 [accepted]
PHST- 2011/07/16 06:00 [entrez]
PHST- 2011/07/16 06:00 [pubmed]
PHST- 2011/12/14 06:00 [medline]
PHST- 2012/10/01 00:00 [pmc-release]
AID - S0142-9612(11)00710-1 [pii]
AID - 10.1016/j.biomaterials.2011.06.045 [doi]
PST - ppublish
SO  - Biomaterials. 2011 Oct;32(30):7503-13. doi: 10.1016/j.biomaterials.2011.06.045. 
      Epub 2011 Jul 14.