PMID- 21757770
OWN - NLM
STAT- MEDLINE
DCOM- 20110929
LR  - 20211020
IS  - 1533-3450 (Electronic)
IS  - 1046-6673 (Print)
IS  - 1046-6673 (Linking)
VI  - 22
IP  - 8
DP  - 2011 Aug
TI  - Kidney dendritic cells induce innate immunity against bacterial pyelonephritis.
PG  - 1435-41
LID - 10.1681/ASN.2010101072 [doi]
AB  - Dendritic cells (DCs) are the most abundant immune cells in the kidney and form 
      an intricate network in the tubulointerstitium, suggesting that they may play an 
      important role in interstitial infections such as pyelonephritis. Here, we 
      optimized a murine pyelonephritis model by instilling uropathogenic Escherichia 
      coli two times at a 3-hour interval, which produced an infection rate of 84%. By 
      3 hours after the second instillation, resident kidney DCs began secreting the 
      chemokine CXCL2, which recruits neutrophilic granulocytes. During the time 
      studied, DCs remained responsible for most of the CXCL2 production. Neutrophils 
      began infiltrating the kidney 3 hours after the second instillation and 
      phagocytozed bacteria. Macrophages followed 3 hours later and contributed much 
      less to both CXCL2 production and bacterial phagocytosis. To investigate whether 
      DCs recruit neutrophils into the kidney for antibacterial defense, we used 
      CD11c.DTR mice allowing conditional depletion of CD11c(+) dendritic cells. The 
      absence of CD11c(+) DCs markedly delayed neutrophil recruitment and bacterial 
      clearance. In conclusion, these findings suggest that the tubulointerstitial 
      dendritic cell network serves an innate immune sentinel function against 
      bacterial pyelonephritis.
FAU - Tittel, Andre P
AU  - Tittel AP
AD  - Institutes of Molecular Medicine and Experimental Immunology (IMMEI), Rheinische 
      Friedrich-Wilhelms-Universita  t,Bonn, Germany.
FAU - Heuser, Christoph
AU  - Heuser C
FAU - Ohliger, Christina
AU  - Ohliger C
FAU - Knolle, Percy A
AU  - Knolle PA
FAU - Engel, Daniel R
AU  - Engel DR
FAU - Kurts, Christian
AU  - Kurts C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110714
PL  - United States
TA  - J Am Soc Nephrol
JT  - Journal of the American Society of Nephrology : JASN
JID - 9013836
RN  - 0 (CD11c Antigen)
RN  - 0 (Virulence Factors)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/biosynthesis
MH  - Dendritic Cells/*cytology/immunology
MH  - Escherichia coli/metabolism
MH  - Humans
MH  - Immunity, Innate
MH  - Incidence
MH  - Kidney/cytology/microbiology/*pathology
MH  - Macrophages/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Phagocytes/cytology
MH  - Pyelonephritis/*immunology/*microbiology
MH  - Urinary Tract/pathology
MH  - Virulence Factors
PMC - PMC3148698
EDAT- 2011/07/16 06:00
MHDA- 2011/10/01 06:00
PMCR- 2012/08/01
CRDT- 2011/07/16 06:00
PHST- 2011/07/16 06:00 [entrez]
PHST- 2011/07/16 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - ASN.2010101072 [pii]
AID - 2010101072 [pii]
AID - 10.1681/ASN.2010101072 [doi]
PST - ppublish
SO  - J Am Soc Nephrol. 2011 Aug;22(8):1435-41. doi: 10.1681/ASN.2010101072. Epub 2011 
      Jul 14.