PMID- 21762399
OWN - NLM
STAT- MEDLINE
DCOM- 20120112
LR  - 20211020
IS  - 1399-0039 (Electronic)
IS  - 0001-2815 (Print)
IS  - 0001-2815 (Linking)
VI  - 78
IP  - 4
DP  - 2011 Oct
TI  - Multiplexed, ligation-dependent probe amplification for rapid and inexpensive 
      HLA-DQB1 allelotyping.
PG  - 275-80
LID - 10.1111/j.1399-0039.2011.01737.x [doi]
AB  - Many effective options exist to accurately type DNA for human leukocyte antigen 
      (HLA) alleles. However, most of the existing methods are excessively costly in 
      terms of overall monetary costs, DNA requirements, and proprietary software. We 
      present a novel assay capable of resolving heterozygous HLA-DQB1 allelotypes at 
      two digits, with even greater specificity for the HLA-DQB1*06 allele family, by 
      using the multiplexed ligation-dependent probe amplification technology. This 
      assay provides more specific allele data than genome-wide analysis and is more 
      affordable than sequencing, making it a useful intermediate for researchers 
      seeking to accurately allelotype human DNA samples.
CI  - (c) 2011 John Wiley & Sons A/S.
FAU - Akers, N K
AU  - Akers NK
AD  - Division of Environmental Health Sciences, School of Public Health, University of 
      California, Berkeley, Berkeley, CA, USA. kipp@berkeley.edu
FAU - Curry, J D
AU  - Curry JD
FAU - Smith, M T
AU  - Smith MT
FAU - Bracci, P M
AU  - Bracci PM
FAU - Skibola, C F
AU  - Skibola CF
LA  - eng
GR  - P42 ES004705/ES/NIEHS NIH HHS/United States
GR  - CA122663/CA/NCI NIH HHS/United States
GR  - R01 CA122663/CA/NCI NIH HHS/United States
GR  - CA104682/CA/NCI NIH HHS/United States
GR  - R01 CA104682/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20110718
PL  - England
TA  - Tissue Antigens
JT  - Tissue antigens
JID - 0331072
RN  - 0 (HLA-DQ beta-Chains)
RN  - 0 (HLA-DQB1 antigen)
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - *Alleles
MH  - Cell Line
MH  - Female
MH  - HLA-DQ beta-Chains/*genetics
MH  - Heterozygote
MH  - Histocompatibility Testing/economics/*methods
MH  - Humans
MH  - Ligase Chain Reaction/economics/*methods
MH  - Male
MH  - Oligonucleotide Probes/*chemistry
PMC - PMC3431962
MID - NIHMS399405
COIS- Conflicts of Interest The authors have no conflicts of interest or commercial 
      links pertinent to this manuscript to report.
EDAT- 2011/07/19 06:00
MHDA- 2012/01/13 06:00
PMCR- 2012/08/31
CRDT- 2011/07/19 06:00
PHST- 2011/07/19 06:00 [entrez]
PHST- 2011/07/19 06:00 [pubmed]
PHST- 2012/01/13 06:00 [medline]
PHST- 2012/08/31 00:00 [pmc-release]
AID - 10.1111/j.1399-0039.2011.01737.x [doi]
PST - ppublish
SO  - Tissue Antigens. 2011 Oct;78(4):275-80. doi: 10.1111/j.1399-0039.2011.01737.x. 
      Epub 2011 Jul 18.