PMID- 21763429
OWN - NLM
STAT- MEDLINE
DCOM- 20111229
LR  - 20110902
IS  - 1095-9572 (Electronic)
IS  - 1053-8119 (Linking)
VI  - 58
IP  - 3
DP  - 2011 Oct 1
TI  - Differential response to specific 5-Ht(7) versus whole-serotonergic drugs in rat 
      forebrains: a phMRI study.
PG  - 885-94
LID - 10.1016/j.neuroimage.2011.06.089 [doi]
AB  - We recently suggested that serotonin 7 (5-Ht7) receptors may play a role in 
      ADHD-like symptoms, at least in animal models. A mixed 5-Ht(1a/7) agonist, 
      8-OH-DPAT, counteracted the augmented levels of basal impulsivity, observed after 
      treatment with a selective 5-Ht7 antagonist, SB269970 (Leo et al., 2009). In the 
      present study, these serotonergic compounds were investigated by pharmacological 
      magnetic resonance imaging (phMRI) at 4.7 T in adult isoflurane-anaesthetized 
      rats. Axial echo-planar images were collected from the prefrontal cortex (PFC), 
      ventral (nucleus accumbens, NAcc) and dorsal (dStr) striata, the hippocampus and 
      the thalamus. After consecutive image collection for 30 min (50 baseline images), 
      adult rats received either SB269970 (3mg/kg), 8-OH-DPAT (0.06 mg/kg) or saline 
      intra-peritoneally (i.p.) via a remote cannula; the images were then collected 
      for further 30 min (50 post-treatment images). Data were analysed 1) through an 
      activation map generated on brain templates, obtained by using animals from each 
      experimental group; 2) by a two-way ANOVA for the evaluation of temporal 
      profiles, extracted within selected ROIs of each animal. Both compounds increased 
      the BOLD signal in the areas of interest: SB269970, the selective 5-Ht7 
      antagonist, induced a significant effect in the PFC, particularly the orbital 
      (oPFC) region, and in the NAcc. This effect started 6 to 12 min after drug 
      administration, reached a maximum (+2.8%/+2.3%) at 12 to 18 min, and then moved 
      to the dorsal thalamic nuclei. In contrast, the effects of 8-OH-DPAT were first 
      observed in midline thalamic nuclei, and later appeared in forebrain regions: its 
      effects were modest and transient within the NAcc and oPFC (+1.7% at 18 to 24 min 
      after injection), whereas they were higher and long-lasting in the dStr and PFC, 
      specifically the medial (mPFC) region (+3.1%/+4.0% from 15 min after drug 
      administration onwards). The brain BOLD changes, reported as a consequence of 
      selective 5-Ht7 antagonist administration, seemed restricted to the oPFC, NAcc 
      and dorso-thalamic circuits, whereas the non-selective blockade of serotonergic 
      receptors affected the mPFC, dStr and mid(line)-thalamic circuitry. The present 
      findings revealed two differential serotonergic sub-pathways, as evidenced by the 
      detection of physiological vascular feedback and/or neuronal activation.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Canese, Rossella
AU  - Canese R
AD  - Department of Cell Biology and Neurosciences, Istituto Superiore di Sanita, Rome, 
      Italy.
FAU - Marco, Eva M
AU  - Marco EM
FAU - De Pasquale, Francesco
AU  - De Pasquale F
FAU - Podo, Franca
AU  - Podo F
FAU - Laviola, Giovanni
AU  - Laviola G
FAU - Adriani, Walter
AU  - Adriani W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110708
PL  - United States
TA  - Neuroimage
JT  - NeuroImage
JID - 9215515
RN  - 0 (Receptors, Serotonin)
RN  - 0 (Serotonin Agents)
RN  - 0 (serotonin 7 receptor)
RN  - 78950-78-4 (8-Hydroxy-2-(di-n-propylamino)tetralin)
SB  - IM
MH  - 8-Hydroxy-2-(di-n-propylamino)tetralin/pharmacology
MH  - Animals
MH  - Brain Mapping/*methods
MH  - Image Processing, Computer-Assisted
MH  - Magnetic Resonance Imaging
MH  - Male
MH  - Neural Pathways/drug effects/metabolism
MH  - Prosencephalon/*drug effects/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Serotonin/*metabolism
MH  - Serotonin Agents/*pharmacology
EDAT- 2011/07/19 06:00
MHDA- 2011/12/30 06:00
CRDT- 2011/07/19 06:00
PHST- 2010/10/08 00:00 [received]
PHST- 2011/06/24 00:00 [revised]
PHST- 2011/06/29 00:00 [accepted]
PHST- 2011/07/19 06:00 [entrez]
PHST- 2011/07/19 06:00 [pubmed]
PHST- 2011/12/30 06:00 [medline]
AID - S1053-8119(11)00754-3 [pii]
AID - 10.1016/j.neuroimage.2011.06.089 [doi]
PST - ppublish
SO  - Neuroimage. 2011 Oct 1;58(3):885-94. doi: 10.1016/j.neuroimage.2011.06.089. Epub 
      2011 Jul 8.