PMID- 21763753
OWN - NLM
STAT- MEDLINE
DCOM- 20120529
LR  - 20220408
IS  - 1872-7972 (Electronic)
IS  - 0304-3940 (Linking)
VI  - 501
IP  - 2
DP  - 2011 Aug 26
TI  - Effects of resibufogenin on voltage-gated sodium channels in cultured rat 
      hippocampal neurons.
PG  - 112-6
LID - 10.1016/j.neulet.2011.06.059 [doi]
AB  - Voltage-gated sodium channels (VGSCs) play important roles in maintaining the 
      excitability of hippocampal neurons. The present study investigated the effects 
      of resibufogenin (RBG, a main component of bufadienolides) on voltage-gated 
      sodium channel currents (I(Na)) in rat hippocampal neurons using whole-cell patch 
      clamp recording. According to the results, RBG activated I(Na) in a 
      concentration-dependent manner. RBG at 1 muM concentration could alter some 
      channel kinetics of I(Na), such as activation thresholds, steady-state activation 
      and inactivation curves, time constant of recovery, and activity-dependent 
      attenuation of I(Na). RBG influenced peak amplitude, overshoot and half-width of 
      the evoked single action potential, and simultaneously lessened the firing rate 
      of evoked repetitive firing. These findings suggested that I(Na) is probably a 
      target of RBG, which may explain the mechanisms for the pathological effects of 
      RBG on central nervous system.
CI  - Copyright (c) 2011 Elsevier Ireland Ltd. All rights reserved.
FAU - Hao, Shuang
AU  - Hao S
AD  - School of Life Science and Biotechnology, Dalian University of Technology, No. 2 
      Linggong Road, Dalian, Liaoning 116024, PR China.
FAU - Bao, Yong-Ming
AU  - Bao YM
FAU - Zhao, Rong-Guo
AU  - Zhao RG
FAU - Wang, He-Shuang
AU  - Wang HS
FAU - Bi, Jing
AU  - Bi J
FAU - An, Li-Jia
AU  - An LJ
FAU - Jiang, Bo
AU  - Jiang B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110708
PL  - Ireland
TA  - Neurosci Lett
JT  - Neuroscience letters
JID - 7600130
RN  - 0 (Bufanolides)
RN  - 0 (Sodium Channel Agonists)
RN  - 0 (Sodium Channels)
RN  - 3K654P2M4J (bufogenin)
SB  - IM
MH  - Action Potentials/drug effects
MH  - Animals
MH  - Bufanolides/*toxicity
MH  - Cells, Cultured
MH  - Hippocampus/*cytology
MH  - Ion Channel Gating/drug effects/physiology
MH  - Medicine, Chinese Traditional/methods
MH  - Neurons/cytology/*drug effects/*physiology
MH  - Patch-Clamp Techniques/methods
MH  - Rats
MH  - Respiratory Mechanics/drug effects
MH  - *Sodium Channel Agonists
MH  - Sodium Channels/*physiology
EDAT- 2011/07/19 06:00
MHDA- 2012/05/30 06:00
CRDT- 2011/07/19 06:00
PHST- 2011/05/05 00:00 [received]
PHST- 2011/06/29 00:00 [revised]
PHST- 2011/06/29 00:00 [accepted]
PHST- 2011/07/19 06:00 [entrez]
PHST- 2011/07/19 06:00 [pubmed]
PHST- 2012/05/30 06:00 [medline]
AID - S0304-3940(11)01014-7 [pii]
AID - 10.1016/j.neulet.2011.06.059 [doi]
PST - ppublish
SO  - Neurosci Lett. 2011 Aug 26;501(2):112-6. doi: 10.1016/j.neulet.2011.06.059. Epub 
      2011 Jul 8.