PMID- 21764030
OWN - NLM
STAT- MEDLINE
DCOM- 20111214
LR  - 20220330
IS  - 1879-1891 (Electronic)
IS  - 0002-9394 (Print)
IS  - 0002-9394 (Linking)
VI  - 152
IP  - 5
DP  - 2011 Nov
TI  - Feasibility of a method for en face imaging of photoreceptor cell integrity.
PG  - 807-14.e1
LID - 10.1016/j.ajo.2011.04.027 [doi]
AB  - PURPOSE: To report a method for en face imaging of the photoreceptor inner and 
      outer segment junction by spectral-domain optical coherence tomography (SD OCT) 
      and to describe findings in normal subjects and patients with various retinal 
      diseases. DESIGN: Observational case series. METHODS: SD OCT images were acquired 
      from 6 normal subjects (mean age, 44 +/- 11 years) and from 5 subjects with retinal 
      diseases (mean age, 66 +/- 22 years). A customized high-density SD OCT volume scan 
      was acquired on the retina. SD OCT B-scan images were segmented automatically to 
      extract intensity data along the inner and outer segment junction. Data obtained 
      from the raster B-scans were combined to generate an inner and outer segment en 
      face image in a 4.4 x 4.4-mm retinal area centered on the fovea. The 
      foveal-to-parafoveal mean intensity ratio was measured, and repeatability was 
      determined. An infrared scanning laser ophthalmoscope image was acquired and was 
      cropped to provide a field of view similar to the inner and outer segment en face 
      image. RESULTS: Inner and outer segment en face images generated in normal 
      subjects provided clear visualization of the retinal vasculature, matching the 
      vascular network observed in the infrared scanning laser ophthalmoscope image. In 
      normal subjects, the foveal-to-parafoveal mean intensity ratio was 0.88 +/- 0.06, 
      and repeatability of measurements was, on average, 7%. In macular hole, a dark 
      circular region was observed in the inner and outer segment en face image, 
      indicative of photoreceptor cell loss. In age-related macular degeneration, the 
      en face image displayed nonuniform texture corresponding to topographic 
      variations in the inner and outer segment junction. In central serous 
      retinopathy, areas of lower intensity were visible on the en face image 
      corresponding to regions of prior neurosensory elevation. In cystoid macular 
      edema, reduced intensity was present in the inner and outer segment en face image 
      in areas with increased retinal thickness. In diabetic retinopathy, the inner and 
      outer segment en face image displayed regions of reduced intensity resulting from 
      edema, laser scars, or both. CONCLUSIONS: Detection of intensity abnormalities in 
      the inner and outer segment en face image is useful for monitoring the integrity 
      of photoreceptor cells in the course of disease progression and therapeutic 
      intervention.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Wanek, Justin
AU  - Wanek J
AD  - Department of Ophthalmology and Visual Sciences, University of Illinois at 
      Chicago, Chicago, Illinois 60612, USA.
FAU - Zelkha, Ruth
AU  - Zelkha R
FAU - Lim, Jennifer I
AU  - Lim JI
FAU - Shahidi, Mahnaz
AU  - Shahidi M
LA  - eng
GR  - EY01792/EY/NEI NIH HHS/United States
GR  - P30 EY001792/EY/NEI NIH HHS/United States
GR  - P30 EY001792-33/EY/NEI NIH HHS/United States
GR  - R01 EY014275-06/EY/NEI NIH HHS/United States
GR  - R01 EY014275/EY/NEI NIH HHS/United States
GR  - EY14275/EY/NEI NIH HHS/United States
PT  - Case Reports
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110720
PL  - United States
TA  - Am J Ophthalmol
JT  - American journal of ophthalmology
JID - 0370500
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Feasibility Studies
MH  - Female
MH  - Humans
MH  - Image Processing, Computer-Assisted/methods
MH  - Male
MH  - Middle Aged
MH  - Retinal Diseases/*diagnosis
MH  - Retinal Photoreceptor Cell Inner Segment/*pathology
MH  - Retinal Photoreceptor Cell Outer Segment/*pathology
MH  - Tomography, Optical Coherence/*methods
MH  - Visual Acuity/physiology
PMC - PMC3200461
MID - NIHMS312119
EDAT- 2011/07/19 06:00
MHDA- 2011/12/15 06:00
PMCR- 2012/11/01
CRDT- 2011/07/19 06:00
PHST- 2011/02/04 00:00 [received]
PHST- 2011/04/26 00:00 [revised]
PHST- 2011/04/28 00:00 [accepted]
PHST- 2011/07/19 06:00 [entrez]
PHST- 2011/07/19 06:00 [pubmed]
PHST- 2011/12/15 06:00 [medline]
PHST- 2012/11/01 00:00 [pmc-release]
AID - S0002-9394(11)00367-9 [pii]
AID - 10.1016/j.ajo.2011.04.027 [doi]
PST - ppublish
SO  - Am J Ophthalmol. 2011 Nov;152(5):807-14.e1. doi: 10.1016/j.ajo.2011.04.027. Epub 
      2011 Jul 20.