PMID- 21764073
OWN - NLM
STAT- MEDLINE
DCOM- 20111013
LR  - 20131121
IS  - 1095-8673 (Electronic)
IS  - 0022-4804 (Linking)
VI  - 170
IP  - 1
DP  - 2011 Sep
TI  - PRP&F/P MPs improved survival of dorsal paired pedicle skin flaps in rats.
PG  - e189-96
LID - 10.1016/j.jss.2011.05.051 [doi]
AB  - BACKGROUND: Skin flap necrosis is a problem encountered postoperatively. The 
      purpose of this study was to evaluate the effects of platelet-rich plasma 
      containing fragmin/protamine microparticles (PRP&F/P MPs) on viability in a rat 
      dorsal paired pedicle skin (DPPS) flap. MATERIALS AND METHODS: Two symmetrical 
      adjoining rectangular flaps (8 x 2 cm each) were drawn on the rat dorsum. Two 
      days after PRP&F/P MPs-, PRP-, F/P MPs-, and saline (control)-injections (n = 8 
      each), flaps were elevated as a random pattern flap without the lateral thoracic, 
      posterior intercostal, and deep circumflex iliac vessels. The flaps were 
      immediately sutured back and the flap survival area was measured 7 d after flap 
      elevation. RESULTS: The flap survival rate in PRP&F/P MPs-injected groups (73.1% 
      +/- 4.2%) was significantly higher than those in PRP (64.9% +/- 4.0%), F/P MPs (59.4 
      +/- 4.5%), and control (61.2% +/- 4.2%) groups. Histologic observation of the flaps 
      showed survived thick granulation tissue and neovascularization in PRP&F/P 
      MPs-injected groups. CONCLUSIONS: When PRP&F/P MPs are administered 2 d before 
      the flap elevation, the improved flap survivals are observed. The pre-injection 
      of PRP&F/P MPs may thus represent a promising treatment to prevent skin flap 
      necrosis in reconstructive surgery.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Takikawa, Megumi
AU  - Takikawa M
AD  - Department of Plastic and Reconstructive Surgery, National Defense Medical 
      College, Saitama, Japan.
FAU - Sumi, Yuki
AU  - Sumi Y
FAU - Ishihara, Masayuki
AU  - Ishihara M
FAU - Kishimoto, Satoko
AU  - Kishimoto S
FAU - Nakamura, Shingo
AU  - Nakamura S
FAU - Yanagibayashi, Satoshi
AU  - Yanagibayashi S
FAU - Hattori, Hidemi
AU  - Hattori H
FAU - Azuma, Ryuichi
AU  - Azuma R
FAU - Yamamoto, Naoto
AU  - Yamamoto N
FAU - Kiyosawa, Tomoharu
AU  - Kiyosawa T
LA  - eng
PT  - Journal Article
DEP - 20110623
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (Protamines)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - S79O08V79F (Dalteparin)
SB  - IM
MH  - Animals
MH  - Dalteparin/*pharmacology
MH  - Male
MH  - *Platelet-Rich Plasma
MH  - Protamines/*pharmacology
MH  - Rats
MH  - Rats, Inbred F344
MH  - Skin/pathology
MH  - Surgical Flaps/*physiology
MH  - Vascular Endothelial Growth Factor A/analysis
EDAT- 2011/07/19 06:00
MHDA- 2011/10/14 06:00
CRDT- 2011/07/19 06:00
PHST- 2011/03/31 00:00 [received]
PHST- 2011/05/16 00:00 [revised]
PHST- 2011/05/25 00:00 [accepted]
PHST- 2011/07/19 06:00 [entrez]
PHST- 2011/07/19 06:00 [pubmed]
PHST- 2011/10/14 06:00 [medline]
AID - S0022-4804(11)00509-9 [pii]
AID - 10.1016/j.jss.2011.05.051 [doi]
PST - ppublish
SO  - J Surg Res. 2011 Sep;170(1):e189-96. doi: 10.1016/j.jss.2011.05.051. Epub 2011 
      Jun 23.