PMID- 21764095
OWN - NLM
STAT- MEDLINE
DCOM- 20120110
LR  - 20110919
IS  - 1532-3072 (Electronic)
IS  - 0040-8166 (Linking)
VI  - 43
IP  - 5
DP  - 2011 Oct
TI  - Genome-wide expression profiling of hepatic oval cells after partial hepatectomy 
      in rats.
PG  - 291-303
LID - 10.1016/j.tice.2011.06.001 [doi]
AB  - To examine the changes of biological activities in hepatic oval cells (HOCs) 
      elicited by 70% partial hepatectomy (PH) and understand the relationship between 
      this cell and liver regeneration (LR), this study isolated and obtained the high 
      purity HOCs (>/= 95%) from rat regenerating livers, and then monitored gene 
      expression profiling of rat hepatic oval cells following surgical operation. 
      Results showed that there were LR-related 1059 genes. These genes were grossly 
      classified into three groups using a fold change cut-off threshold of three-fold: 
      up-regulation, down-regulation and up/down regulation. Analyses of gene 
      expression patterns combined with gene functional categorization suggested that 
      genes in the categories "nucleic acid metabolism" and "cell cycle" were dominated 
      by up-regulated expression. Genes in the functional groups "cell metabolism" and 
      "oxidation reduction" were significantly enriched in expression pattern 
      characterized by down-regulation. According to above mentioned results, the 
      synchronized induction of DNA replication and cell proliferation-involved genes 
      suggested that the peak of oval cell proliferation might occur between 30 and 36 
      h post-PH. The amino acid transformation-involved genes were down-regulated at 
      the early phase of LR, which perhaps trigger the storage of those amino acids 
      essential for protein synthesis. Reduced oxidative-reduction activity at early 
      phase might be related to negative influence of surgical operation on its 
      detoxification capacity. Conclusively, the genome-wide transcriptional analysis 
      of oval cells would contribute to our understanding of the nature of LR at cell 
      level.
CI  - Copyright (c) 2011. Published by Elsevier Ltd.
FAU - Xu, Cunshuan
AU  - Xu C
AD  - College of Life Science, Henan Normal University, Xinxiang 453007, China. 
      cellkeylab@126.com
FAU - Chen, Xiaoguang
AU  - Chen X
FAU - Chang, Cuifang
AU  - Chang C
FAU - Wang, Gaiping
AU  - Wang G
FAU - Wang, Wenbo
AU  - Wang W
FAU - Zhang, Lianxing
AU  - Zhang L
FAU - Zhu, Qiushi
AU  - Zhu Q
FAU - Wang, Lei
AU  - Wang L
FAU - Zhang, Fuchun
AU  - Zhang F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110720
PL  - Scotland
TA  - Tissue Cell
JT  - Tissue & cell
JID - 0214745
RN  - 0 (Cell Cycle Proteins)
SB  - IM
MH  - Animals
MH  - Cell Cycle Proteins/genetics/metabolism
MH  - Cell Differentiation
MH  - Cell Survival
MH  - Cluster Analysis
MH  - Down-Regulation
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - *Hepatectomy
MH  - Hepatocytes/*cytology/physiology
MH  - Immunohistochemistry
MH  - Liver/cytology/physiology
MH  - *Liver Regeneration
MH  - Oligonucleotide Array Sequence Analysis
MH  - Oxidation-Reduction
MH  - Protein Biosynthesis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
MH  - Up-Regulation
EDAT- 2011/07/19 06:00
MHDA- 2012/01/11 06:00
CRDT- 2011/07/19 06:00
PHST- 2011/01/12 00:00 [received]
PHST- 2011/06/03 00:00 [revised]
PHST- 2011/06/06 00:00 [accepted]
PHST- 2011/07/19 06:00 [entrez]
PHST- 2011/07/19 06:00 [pubmed]
PHST- 2012/01/11 06:00 [medline]
AID - S0040-8166(11)00065-6 [pii]
AID - 10.1016/j.tice.2011.06.001 [doi]
PST - ppublish
SO  - Tissue Cell. 2011 Oct;43(5):291-303. doi: 10.1016/j.tice.2011.06.001. Epub 2011 
      Jul 20.