PMID- 21764284
OWN - NLM
STAT- MEDLINE
DCOM- 20130624
LR  - 20120420
IS  - 1873-4847 (Electronic)
IS  - 0955-2863 (Linking)
VI  - 23
IP  - 5
DP  - 2012 May
TI  - 10e12z CLA alters adipocyte differentiation and adipocyte cytokine expression and 
      induces macrophage proliferation.
PG  - 510-8
LID - 10.1016/j.jnutbio.2011.02.009 [doi]
AB  - The trans-10, cis-12 (10e12z) conjugated linoleic acid (CLA) isomer of CLA is 
      responsible for loss of lipid storage or adipose tissue in vitro or in vivo. This 
      isomer also induces inflammatory signaling in both mouse and human adipocytes in 
      vitro. However, when these events occur and whether they are significant enough 
      to affect other cell types are unclear. In these experiments, the 3T3-L1 cell 
      line has been used to examine the interaction between inflammatory signaling and 
      decreased differentiation or lipid storage induced by 10e12z CLA. In assays 
      measuring both lipid accumulation and gene expression, differentiating 3T3-L1 
      cells exhibit concurrent induction of inflammatory signaling, as measured by 
      cyclooxygenase-2 expression, and a decrease in adipocyte marker gene expression. 
      Furthermore, in fully differentiated adipocytes, as identified in microarray 
      assays and confirmed with real-time polymerase chain reaction, 10e12z CLA also 
      significantly affected expression of both matrix metalloprotein-3 (MMP-3), 
      collagen VI alpha 3 ColVI alpha 3 (VIalpha3) and the cytokine epiregulin, demonstrating 
      that the effects of 10e12z broadly impact adipocyte function. In agreement with 
      other experimental systems, 10e12z CLA inhibited RAW 264.7 cell proliferation; 
      however, in response to adipocyte-conditioned media, 10e12z-CLA-treated 
      adipocytes induced proliferation of this cell line, suggesting that the effect of 
      10e12z CLA is context dependent. These results are largely consistent with the 
      known activation of the inflammatory mediator nuclear factor-kappaB in adipocytes in 
      vitro and in vivo by 10e12z CLA treatment and demonstrate that adipose is an 
      important target tissue of this isomer that impacts other cell types.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Belda, Benjamin J
AU  - Belda BJ
AD  - The Department of Veterinary and Biomedical Sciences, The Pennsylvania State 
      University, University Park, PA 16802, USA.
FAU - Thompson, Jerry T
AU  - Thompson JT
FAU - Eser, Pinar O
AU  - Eser PO
FAU - Vanden Heuvel, John P
AU  - Vanden Heuvel JP
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20110720
PL  - United States
TA  - J Nutr Biochem
JT  - The Journal of nutritional biochemistry
JID - 9010081
RN  - 0 (Cytokines)
RN  - 0 (Linoleic Acids, Conjugated)
RN  - 0 (trans-10,cis-12-conjugated linoleic acid)
RN  - EC 1.14.99.1 (Cyclooxygenase 2)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/cytology/*drug effects/metabolism
MH  - Animals
MH  - *Cell Differentiation
MH  - Cyclooxygenase 2/genetics/metabolism
MH  - Cytokines/*metabolism
MH  - Linoleic Acids, Conjugated/*pharmacology
MH  - Macrophages/cytology/*drug effects/metabolism
MH  - Mice
MH  - Stereoisomerism
EDAT- 2011/07/19 06:00
MHDA- 2013/06/26 06:00
CRDT- 2011/07/19 06:00
PHST- 2010/11/12 00:00 [received]
PHST- 2011/02/16 00:00 [revised]
PHST- 2011/02/17 00:00 [accepted]
PHST- 2011/07/19 06:00 [entrez]
PHST- 2011/07/19 06:00 [pubmed]
PHST- 2013/06/26 06:00 [medline]
AID - S0955-2863(11)00082-9 [pii]
AID - 10.1016/j.jnutbio.2011.02.009 [doi]
PST - ppublish
SO  - J Nutr Biochem. 2012 May;23(5):510-8. doi: 10.1016/j.jnutbio.2011.02.009. Epub 
      2011 Jul 20.