PMID- 21764481
OWN - NLM
STAT- MEDLINE
DCOM- 20121024
LR  - 20240417
IS  - 1558-1497 (Electronic)
IS  - 0197-4580 (Print)
IS  - 0197-4580 (Linking)
VI  - 33
IP  - 8
DP  - 2012 Aug
TI  - Prevention of age-related changes in hippocampal levels of 5-methylcytidine by 
      caloric restriction.
PG  - 1672-81
LID - 10.1016/j.neurobiolaging.2011.06.003 [doi]
AB  - Aberrant DNA methylation patterns have been linked to molecular and cellular 
      alterations in the aging brain. Caloric restriction (CR) and upregulation of 
      antioxidants have been proposed as interventions to prevent or delay age-related 
      brain pathology. Previously, we have shown in large cohorts of aging mice, that 
      age-related increases in DNA methyltransferase 3a (Dnmt3a) immunoreactivity in 
      the mouse hippocampus were attenuated by CR, but not by overexpression of 
      superoxide dismutase 1 (SOD1). Here, we investigated age-related alterations of 
      5-methylcytidine (5-mC), a marker of DNA methylation levels, in a hippocampal 
      subregion-specific manner. Examination of 5-mC immunoreactivity in 12- and 
      24-month-old wild type (WT) mice on control diet, mice overexpressing SOD1 on 
      control diet, wild type mice on CR, and SOD1 mice on CR, indicated an age-related 
      increase in 5-mC immunoreactivity in the hippocampal dentate gyrus, CA3, and 
      CA1-2 regions, which was prevented by CR but not by SOD1 overexpression. 
      Moreover, positive correlations between 5-mC and Dnmt3a immunoreactivity were 
      observed in the CA3 and CA1-2. These findings suggest a crucial role for DNA 
      methylation in hippocampal aging and in the mediation of the beneficial effects 
      of CR on aging.
CI  - Copyright (c) 2012 Elsevier Inc. All rights reserved.
FAU - Chouliaras, Leonidas
AU  - Chouliaras L
AD  - School for Mental Health and Neuroscience, Department of Psychiatry and 
      Neuropsychology, Faculty of Health, Medicine and Life Sciences, European Graduate 
      School of Neuroscience, Maastricht University Medical Centre, Maastricht, The 
      Netherlands.
FAU - van den Hove, Daniel L A
AU  - van den Hove DL
FAU - Kenis, Gunter
AU  - Kenis G
FAU - Keitel, Stella
AU  - Keitel S
FAU - Hof, Patrick R
AU  - Hof PR
FAU - van Os, Jim
AU  - van Os J
FAU - Steinbusch, Harry W M
AU  - Steinbusch HW
FAU - Schmitz, Christoph
AU  - Schmitz C
FAU - Rutten, Bart P F
AU  - Rutten BP
LA  - eng
GR  - P50 AG005138/AG/NIA NIH HHS/United States
GR  - P50 AG005138-27/AG/NIA NIH HHS/United States
GR  - P50AG05138/AG/NIA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20110718
PL  - United States
TA  - Neurobiol Aging
JT  - Neurobiology of aging
JID - 8100437
RN  - 0 (Dnmt3a protein, mouse)
RN  - 5CSZ8459RP (Cytidine)
RN  - EC 1.15.1.1 (Sod1 protein, mouse)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.15.1.1 (Superoxide Dismutase-1)
RN  - EC 2.1.1.37 (DNA Methyltransferase 3A)
RN  - TL9PB228DC (5-methylcytidine)
SB  - IM
MH  - Aging/*metabolism
MH  - Animals
MH  - Caloric Restriction/*methods
MH  - Cytidine/*analogs & derivatives/metabolism
MH  - *DNA Methylation
MH  - DNA Methyltransferase 3A
MH  - Hippocampus/*metabolism
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Superoxide Dismutase/genetics/*metabolism
MH  - Superoxide Dismutase-1
PMC - PMC3355211
MID - NIHMS351427
COIS- Disclosure statement The authors declare no actual or potential conflicts of 
      interest relating to this work. All experiments were conducted in compliance with 
      the Principles of Laboratory Animal Care (NIH publication no. 86-23, revised 
      1985) and the Dutch animal protection law, considering international standards. 
      The experimental protocols were approved by the Animal Subjects Ethics Committee 
      of The Maastricht University.
EDAT- 2011/07/19 06:00
MHDA- 2012/10/25 06:00
PMCR- 2012/08/01
CRDT- 2011/07/19 06:00
PHST- 2011/01/26 00:00 [received]
PHST- 2011/05/24 00:00 [revised]
PHST- 2011/06/04 00:00 [accepted]
PHST- 2011/07/19 06:00 [entrez]
PHST- 2011/07/19 06:00 [pubmed]
PHST- 2012/10/25 06:00 [medline]
PHST- 2012/08/01 00:00 [pmc-release]
AID - S0197-4580(11)00212-0 [pii]
AID - 10.1016/j.neurobiolaging.2011.06.003 [doi]
PST - ppublish
SO  - Neurobiol Aging. 2012 Aug;33(8):1672-81. doi: 
      10.1016/j.neurobiolaging.2011.06.003. Epub 2011 Jul 18.