PMID- 21769480
OWN - NLM
STAT- MEDLINE
DCOM- 20120628
LR  - 20211020
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 39
IP  - 4
DP  - 2012 Apr
TI  - HIF-1alpha mRNA levels in Eurasian perch (Perca fluviatilis) exposed to acute and 
      chronic hypoxia.
PG  - 4009-15
LID - 10.1007/s11033-011-1181-8 [doi]
AB  - The big advantage of using molecular biomarkers to monitor oxygen levels in 
      aquatic systems is that responses at the molecular level tend to be more 
      sensitive, and usually occur earlier than those at higher levels of biological 
      organization Aquatic hypoxia is a frequent event, which can occur naturally in a 
      variety of marine, estuarine and freshwater habitats. More often, however, 
      hypoxia arises as a result of euthrophication of aquatic ecosystem and can lead 
      to changes in community structure by eliminating hypoxia-sensitive species. 
      Consequently fish have develop various physiological and biochemical mechanisms 
      to cope with this environmental stress. Many of these adjustments depend to 
      changes in expression of a wide range of genes. The transcriptional responses to 
      hypoxia are primarily mediated by hypoxia-inducible factor-1 (HIF-1), a 
      heterodimer composed of an alpha and beta subunit. This study investigated if HIF-1alpha 
      mRNA levels were regulated by hypoxia in Eurasian perch (Perca fluviatilis), a 
      hypoxia-sensitive fresh water species. The real-time PCR was utilized to monitor 
      dynamic changes in levels of HIF-1alpha mRNA in response to acute (DO 0.4 +/- 0.1 mg/l 
      for 1 h) and chronic (DO 2.8 +/- 0.3 mg/l for 15 days) hypoxia. Our results 
      indicated an up-regulation of HIF-1alpha in brain and liver, but not in muscle tissue 
      after acute hypoxic treatment, whereas significant changes of HIF-1alpha mRNA levels 
      were detected in muscle, but not in brain and liver after chronic hypoxia 
      exposure. This study suggests that HIF-1alpha mRNA level in selected perch tissues 
      could be an useful indicator of acute exposure to hypoxia.
FAU - Rimoldi, Simona
AU  - Rimoldi S
AD  - Department of Biotechnology and Molecular Sciences (DBSM), University of 
      Insubria, via J. H. Dunant, 3, 21100, Varese, Italy. simona.rimoldi@uninsubria.it
FAU - Terova, Genciana
AU  - Terova G
FAU - Ceccuzzi, Pietro
AU  - Ceccuzzi P
FAU - Marelli, Stefano
AU  - Marelli S
FAU - Antonini, Micaela
AU  - Antonini M
FAU - Saroglia, Marco
AU  - Saroglia M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110719
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - 0 (DNA, Complementary)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (RNA, Messenger)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Asia
MH  - DNA, Complementary/biosynthesis
MH  - Europe
MH  - Female
MH  - *Gene Expression Regulation
MH  - Hypoxia/*genetics
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/*genetics/metabolism
MH  - Male
MH  - Organ Specificity/genetics
MH  - Oxygen/metabolism
MH  - Perches/*genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Reference Standards
MH  - Solubility
EDAT- 2011/07/20 06:00
MHDA- 2012/06/29 06:00
CRDT- 2011/07/20 06:00
PHST- 2011/01/14 00:00 [received]
PHST- 2011/07/06 00:00 [accepted]
PHST- 2011/07/20 06:00 [entrez]
PHST- 2011/07/20 06:00 [pubmed]
PHST- 2012/06/29 06:00 [medline]
AID - 10.1007/s11033-011-1181-8 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2012 Apr;39(4):4009-15. doi: 10.1007/s11033-011-1181-8. Epub 2011 
      Jul 19.