PMID- 21773845
OWN - NLM
STAT- MEDLINE
DCOM- 20111125
LR  - 20230722
IS  - 1868-8500 (Electronic)
IS  - 1868-8497 (Print)
IS  - 1868-8497 (Linking)
VI  - 2
IP  - 4
DP  - 2011 Aug
TI  - Novel approaches to quantify estradiol-induced loss of ERbeta1 protein in older 
      mouse ovarian surface epithelium: new tools to assess the role of ER protein 
      subtypes in predisposing to ovarian epithelial cancer?
PG  - 204-13
LID - 10.1007/s12672-011-0077-3 [doi]
AB  - Loss of estrogen receptor-beta (ERbeta) occurs in ovarian epithelial cancer (OEC), a 
      cancer of mainly older women. OEC is linked epidemiologically to hormone 
      replacement therapy, predominantly with estrogen-only formulations. This study 
      introduces a novel, non-biased method to quantify levels of estradiol-induced 
      loss of ERbeta1 protein, and defines, for the first time, normal OSE expression 
      patterns for ERalpha and ERbeta1 with advancing age. Older (7-10 months) Swiss Webster 
      mice were injected with estradiol valerate (EV) while age-matched diestrous 
      controls received oil. Mice were culled after 48 h, and blood and one ovary were 
      frozen for estradiol RIA. Contralateral ovaries were paraffin-embedded for 
      immunohistochemistry. Subsets of serial sections, triple-labeled with 
      immunofluroescent tags, were imaged with confocal microscopy to provide optimal 
      visualization of ER protein subtype expression in OSE. Immunofluorescence 
      emission profiles distinct to ERbeta1 in OSE were standardized and quantified in 
      control mice then compared to profiles from EV-exposed mice. Estradiol levels 
      were significantly elevated in EV-treated mice, both in blood (p < 0.0001) and 
      ovarian tissue (p < 0.001), resulting in 11-fold reduction in OSE expression of 
      ERbeta1 protein (p < 0.0001). In aging OSE, expression patterns of both ER subtypes 
      varied within cells and with cell shape. ER co-localization appeared 
      predominantly cytoplasmic and was infrequent in columnar compared to 
      cuboidal-shaped OSE cells. Immunofluorescence emission profiling and 
      multiple-label immunofluorescent tagging of ER using confocal microscopy, 
      provides sharp definition of ER locus enabling concurrent qualitative and 
      quantitative analysis of ER protein. It offers significant potential for 
      assessing ER protein subtype status in predisposition to OEC.
FAU - Gulliver, Linda S M
AU  - Gulliver LS
AD  - Faculty of Medicine, University of Otago, Dunedin, New Zealand. 
      linda.gulliver@otago.ac.nz
FAU - Hurst, Peter R
AU  - Hurst PR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Horm Cancer
JT  - Hormones & cancer
JID - 101518427
RN  - 0 (Estrogen Receptor beta)
RN  - 0 (Estrogens)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - Aging/*physiology
MH  - Animals
MH  - Carcinoma, Ovarian Epithelial
MH  - Estradiol/*pharmacology
MH  - Estrogen Receptor beta/*metabolism
MH  - Estrogens/*pharmacology
MH  - Female
MH  - Fluorescent Antibody Technique
MH  - Immunohistochemistry
MH  - Mice
MH  - Microscopy, Confocal
MH  - Neoplasms, Glandular and Epithelial/*metabolism
MH  - Ovarian Neoplasms/*metabolism
MH  - Ovary/drug effects/*metabolism
MH  - Radioimmunoassay
PMC - PMC10358137
EDAT- 2011/07/21 06:00
MHDA- 2011/12/13 00:00
PMCR- 2011/07/20
CRDT- 2011/07/21 06:00
PHST- 2011/07/21 06:00 [entrez]
PHST- 2011/07/21 06:00 [pubmed]
PHST- 2011/12/13 00:00 [medline]
PHST- 2011/07/20 00:00 [pmc-release]
AID - 77 [pii]
AID - 10.1007/s12672-011-0077-3 [doi]
PST - ppublish
SO  - Horm Cancer. 2011 Aug;2(4):204-13. doi: 10.1007/s12672-011-0077-3.