PMID- 21774449
OWN - NLM
STAT- MEDLINE
DCOM- 20110809
LR  - 20170214
IS  - 0003-4894 (Print)
IS  - 0003-4894 (Linking)
VI  - 120
IP  - 6
DP  - 2011 Jun
TI  - Implantation of an atelocollagen sponge with autologous bone marrow-derived 
      mesenchymal stromal cells for treatment of vocal fold scarring in a canine model.
PG  - 401-8
AB  - OBJECTIVES: Vocal fold scarring remains a therapeutic challenge. A new 
      regenerative approach is needed to restore disorganized extracellular matrix. 
      Tissue regeneration requires appropriate cells and a scaffold. Bone 
      marrow-derived mesenchymal stromal cells (BMSCs) are multipotent and secrete many 
      kinds of growth factors to regenerate tissues. Atelocollagen sponges have many 
      large pores that permit cell entry. The present study was performed to evaluate 
      whether implantation of an atelocollagen sponge plus BMSCs is effective for the 
      treatment of vocal fold scarring. METHODS: Twelve beagles underwent implantation 
      of an atelocollagen sponge or of an atelocollagen sponge with autologous BMSCs 
      (1.0 x 10(6) cells) in the subepithelial pockets of scarred vocal folds. Six 
      months after the operation, vibratory examinations and histologic examinations 
      were performed. RESULTS: Mucosal vibrations improved significantly for the 
      atelocollagen sponge-implanted vocal folds. Histologic analyses revealed 
      favorable restoration of the extracellular matrix in the lamina propria. 
      Increased distribution of hyaluronic acid and decreased dense collagen deposition 
      were also noted. These improvements were enhanced by implantation of BMSCs. 
      CONCLUSIONS: Implantation of atelocollagen sponges with autologous BMSCs into 
      scarred vocal folds significantly increased hyaluronic acid distribution and 
      decreased dense collagen deposition in the lamina propria, leading to better 
      mucosal vibration.
FAU - Ohno, Satoshi
AU  - Ohno S
AD  - Department of Otolaryngology-Head and Neck Surgery, Graduate School of Medicine, 
      Kyoto University, Kyoto, Japan.
FAU - Hirano, Shigeru
AU  - Hirano S
FAU - Kanemaru, Shin-Ichi
AU  - Kanemaru S
FAU - Kitani, Yoshiharu
AU  - Kitani Y
FAU - Kojima, Tsuyoshi
AU  - Kojima T
FAU - Tateya, Ichiro
AU  - Tateya I
FAU - Nakamura, Tatsuo
AU  - Nakamura T
FAU - Ito, Juichi
AU  - Ito J
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Ann Otol Rhinol Laryngol
JT  - The Annals of otology, rhinology, and laryngology
JID - 0407300
RN  - 0 (Drug Carriers)
RN  - 0 (atelocollagen)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Cicatrix/pathology/*surgery
MH  - *Collagen
MH  - Disease Models, Animal
MH  - Dogs
MH  - Drug Carriers
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - *Surgical Sponges
MH  - Transplantation, Autologous
MH  - Treatment Outcome
MH  - Vocal Cords/pathology/physiopathology/*surgery
MH  - Voice Disorders/pathology/physiopathology/*surgery
EDAT- 2011/07/22 06:00
MHDA- 2011/08/10 06:00
CRDT- 2011/07/22 06:00
PHST- 2011/07/22 06:00 [entrez]
PHST- 2011/07/22 06:00 [pubmed]
PHST- 2011/08/10 06:00 [medline]
AID - 10.1177/000348941112000610 [doi]
PST - ppublish
SO  - Ann Otol Rhinol Laryngol. 2011 Jun;120(6):401-8. doi: 10.1177/000348941112000610.