PMID- 2177516
OWN - NLM
STAT- MEDLINE
DCOM- 19910305
LR  - 20191029
IS  - 0882-4010 (Print)
IS  - 0882-4010 (Linking)
VI  - 9
IP  - 2
DP  - 1990 Aug
TI  - Inhibition of polymorphonuclear leukocyte function by Legionella pneumophila 
      exoproducts.
PG  - 117-25
AB  - Total exoproducts (relative molecular mass greater than 10,000) from wild-type 
      strains of Legionella pneumophila markedly inhibited human polymorphonuclear 
      leukocyte (PMN) superoxide anion generation, at sub-lethal concentrations, in 
      response to four stimuli [1.7, 0, 0.6 and 3.4% of control for zymosan activated 
      particles (ZAP), phorbol myristate acetate (PMA), calcium ionophore (A 23187), 
      and formyl-methionyl-leucyl-phenylalanine (fMLP), respectively]. PMN chemotaxis 
      towards fMLP and spontaneous migration, were also dramatically inhibited (2.8 and 
      2.9% of buffer-treated controls, respectively). In contrast, total exoproducts 
      from the cas-1 strain of L. pneumophila, a protease-deficient mutant generated by 
      ethyl methane sulfonate mutagenesis, failed to inhibit PMN superoxide production 
      in response to ZAP and PMA and only partially inhibited PMN response to A 23187 
      and fMLP. PMN spontaneous migration was unaffected by treatment with total 
      exoproducts from the mutant, while directed chemotaxis was partially inhibited 
      (51.4%). These data demonstrated that L. pneumophila total exoproducts, primarily 
      protease had significant inhibitory effects on normal PMN function and may play 
      an important contributory role in the pathogenesis of legionnaire's disease.
FAU - Sahney, N N
AU  - Sahney NN
AD  - Department of Microbiology and Immunology, School of Medicine, University of 
      Louisville, Kentucky 40292.
FAU - Lambe, B C
AU  - Lambe BC
FAU - Summersgill, J T
AU  - Summersgill JT
FAU - Miller, R D
AU  - Miller RD
LA  - eng
GR  - AI22139/AI/NIAID NIH HHS/United States
GR  - AI23567/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Microb Pathog
JT  - Microbial pathogenesis
JID - 8606191
RN  - 11062-77-4 (Superoxides)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - 9H154DI0UP (Ethyl Methanesulfonate)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Calcimycin/pharmacology
MH  - Chemotaxis, Leukocyte
MH  - Ethyl Methanesulfonate/pharmacology
MH  - Legionella/*metabolism
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Neutrophils/*metabolism
MH  - Superoxides/metabolism
MH  - Tetradecanoylphorbol Acetate/pharmacology
EDAT- 1990/08/01 00:00
MHDA- 1990/08/01 00:01
CRDT- 1990/08/01 00:00
PHST- 1990/08/01 00:00 [pubmed]
PHST- 1990/08/01 00:01 [medline]
PHST- 1990/08/01 00:00 [entrez]
AID - 0882-4010(90)90085-5 [pii]
AID - 10.1016/0882-4010(90)90085-5 [doi]
PST - ppublish
SO  - Microb Pathog. 1990 Aug;9(2):117-25. doi: 10.1016/0882-4010(90)90085-5.