PMID- 21777464
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20111110
LR  - 20211020
IS  - 1756-0500 (Electronic)
IS  - 1756-0500 (Linking)
VI  - 4
DP  - 2011 Jul 21
TI  - Dendritic Cells Activate and Mature after Infection with Mycobacterium 
      tuberculosis.
PG  - 247
LID - 10.1186/1756-0500-4-247 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) can take up an array of different antigens, 
      including microorganisms which they can process and present more effectively than 
      any other antigen presenting cell. However, whether the interaction between the 
      human DC and Mycobacterium tuberculosis represents a defense mechanism by the 
      invaded host, or helping the invader to evade the defense mechanism of the host 
      is still not clearly understood. FINDINGS: To analyze the interactions between M. 
      tuberculosis and immune cells, human peripheral blood monocyte-derived immature 
      DCs were infected with M. tuberculosis H37Rv wild type strain and flow cytometry 
      was used to analyse cell surface expression markers. The ability of the M. 
      tuberculosis infected DC to induce T cell proliferation using 5 and 
      6-carboxyfluorescein diacetate succinimidyl ester (CFSE) dilution technique was 
      also investigated. DCs were found to internalize the mycobacteria and show dose 
      dependent infection and necrosis with different multiplicity of infection. Flow 
      cytometry analysis of cell surface expression markers CD40, CD54, CD80, CD83, 
      CD86 and HLA DR in infected DC revealed significant (p < 0.05) up regulation 
      following infection with M. tuberculosis in comparison to immature DC with no 
      stimulation. Lipopolysaccharide (LPS) from Salmonella abortus equi, a known DC 
      maturation agent, was used as a positive control and showed a comparable up 
      regulation of cell surface markers as observed with M. tuberculosis infected DC. 
      It was revealed that the M. tuberculosis infected DC induced T cell 
      proliferation. CONCLUSION: These data clearly demonstrate that M. tuberculosis 
      induces activation and maturation of human monocyte-derived immature DC as well 
      as induces T cell proliferation in vitro.
FAU - Mihret, Adane
AU  - Mihret A
AD  - Armauer Hansen Research Institute (AHRI), Addis Ababa, Ethiopia. 
      adane_mihret@yahoo.com.
FAU - Mamo, Gezahagne
AU  - Mamo G
FAU - Tafesse, Mesfin
AU  - Tafesse M
FAU - Hailu, Asrat
AU  - Hailu A
FAU - Parida, Shreemanta
AU  - Parida S
LA  - eng
PT  - Journal Article
DEP - 20110721
PL  - England
TA  - BMC Res Notes
JT  - BMC research notes
JID - 101462768
PMC - PMC3148562
EDAT- 2011/07/23 06:00
MHDA- 2011/07/23 06:01
PMCR- 2011/07/21
CRDT- 2011/07/23 06:00
PHST- 2011/06/03 00:00 [received]
PHST- 2011/07/21 00:00 [accepted]
PHST- 2011/07/23 06:00 [entrez]
PHST- 2011/07/23 06:00 [pubmed]
PHST- 2011/07/23 06:01 [medline]
PHST- 2011/07/21 00:00 [pmc-release]
AID - 1756-0500-4-247 [pii]
AID - 10.1186/1756-0500-4-247 [doi]
PST - epublish
SO  - BMC Res Notes. 2011 Jul 21;4:247. doi: 10.1186/1756-0500-4-247.