PMID- 21777583
OWN - NLM
STAT- MEDLINE
DCOM- 20120203
LR  - 20131121
IS  - 1096-0007 (Electronic)
IS  - 0014-4835 (Linking)
VI  - 93
IP  - 5
DP  - 2011 Nov
TI  - Simvastatin upregulates Bcl-2 expression and protects retinal neurons from early 
      ischemia/reperfusion injury in the rat retina.
PG  - 580-5
LID - 10.1016/j.exer.2011.07.003 [doi]
AB  - Simvastatin has been shown to enhance the survival of retinal ganglion cells 
      (RGCs) following ischemia-reperfusion (IR) injury by mediating the expression of 
      stress proteins. The purpose of this study was to investigate the effect of 
      simvastatin on retinal neurons and the expression of apoptotic proteins in a rat 
      IR model. Wistar rats received intravitreal injection of simvastatin immediately 
      after retinal reperfusion. Retinal ischemia was induced by increasing intraocular 
      pressure to 150 mm Hg for 60 min. The number of viable RGCs was measured after 
      retrograde labeling with Fluoro-Gold. Ischemia-induced apoptotic cell death was 
      studied using terminal deoxynucleotidyl transferase-mediated dUTP nick end 
      labeling (TUNEL). We found that simvastatin treatment enhanced RGC survival after 
      retinal ischemia by approximately 40% and decreased retinal neuronal apoptosis. 
      Using western blot analysis, we found that simvastatin upregulated the expression 
      of Bcl-2 in the retina. In contrast, the level of the protein Bax was unaffected 
      by simvastatin treatment. Our results suggest that RGC loss induced by retinal IR 
      may be prevented by simvastatin and that the mechanism underlying this process 
      possibly involves an alteration in the apoptotic pathway.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Ko, Mei-Lan
AU  - Ko ML
AD  - Department of Ophthalmology, General Hsin-Chu Hospital, No. 25, Alley 442, 
      Section One, Chin- Kuo Road, Hisnchu, Taiwan. aaddch@gmail.com
FAU - Chen, Chau-Fong
AU  - Chen CF
FAU - Peng, Pai-Huei
AU  - Peng PH
FAU - Peng, Yi-Hao
AU  - Peng YH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110718
PL  - England
TA  - Exp Eye Res
JT  - Experimental eye research
JID - 0370707
RN  - 0 (2-hydroxy-4,4'-diamidinostilbene, methanesulfonate salt)
RN  - 0 (Bax protein, rat)
RN  - 0 (Hydroxymethylglutaryl-CoA Reductase Inhibitors)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (Stilbamidines)
RN  - 0 (bcl-2-Associated X Protein)
RN  - AGG2FN16EV (Simvastatin)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Blotting, Western
MH  - Cell Count
MH  - Cell Survival/drug effects
MH  - Disease Models, Animal
MH  - Female
MH  - Hydroxymethylglutaryl-CoA Reductase Inhibitors/administration & 
      dosage/*pharmacology
MH  - In Situ Nick-End Labeling
MH  - Intravitreal Injections
MH  - Neuroprotective Agents/administration & dosage/*pharmacology
MH  - Proto-Oncogene Proteins c-bcl-2/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Reperfusion Injury/metabolism/*prevention & control
MH  - Retinal Diseases/metabolism/*prevention & control
MH  - Retinal Ganglion Cells/*drug effects/metabolism
MH  - Simvastatin/administration & dosage/*pharmacology
MH  - Stilbamidines/metabolism
MH  - Up-Regulation
MH  - bcl-2-Associated X Protein/metabolism
EDAT- 2011/07/23 06:00
MHDA- 2012/02/04 06:00
CRDT- 2011/07/23 06:00
PHST- 2011/01/10 00:00 [received]
PHST- 2011/04/23 00:00 [revised]
PHST- 2011/07/06 00:00 [accepted]
PHST- 2011/07/23 06:00 [entrez]
PHST- 2011/07/23 06:00 [pubmed]
PHST- 2012/02/04 06:00 [medline]
AID - S0014-4835(11)00216-8 [pii]
AID - 10.1016/j.exer.2011.07.003 [doi]
PST - ppublish
SO  - Exp Eye Res. 2011 Nov;93(5):580-5. doi: 10.1016/j.exer.2011.07.003. Epub 2011 Jul 
      18.