PMID- 21777684
OWN - NLM
STAT- MEDLINE
DCOM- 20120111
LR  - 20221207
IS  - 1095-8274 (Electronic)
IS  - 1075-9964 (Linking)
VI  - 17
IP  - 5
DP  - 2011 Oct
TI  - In vitro mutagen binding and antimutagenic activity of human Lactobacillus 
      rhamnosus 231.
PG  - 217-22
LID - 10.1016/j.anaerobe.2011.07.001 [doi]
AB  - In vitro mutagen binding ability of human Lactobacillus rhamnosus 231 (Lr 231) 
      was evaluated against acridine orange (AO), N-methyl-N'-nitro-N-nitrosoguanidine 
      (MNNG), 2-amino-3, 8-dimethylimidazo-[4,5-f]-quinoxaline (MeIQx) and 
      4-nitro-o-phenylenediamine (NPD). Binding of AO by Lr 231 is due to adsorption, 
      thereby leading to removal of mutagen in solution and is instantaneous, pH- and 
      concentration-dependent. Whereas, binding of MNNG and MeIQx by Lr 231 results 
      into biotransformation leading to detoxification with subsequent loss of 
      mutagenicity as determined by spectral analysis, thin layer chromatography and 
      Ames test. Binding of mutagen by Lr 231 was dependent on culture age and optimum 
      binding of AO, MNNG and MeIQx was observed to occur with 24 h old culture. Cells 
      of Lr 231 were subjected to different chemical treatments prior to binding 
      studies. Results indicated cell wall component such as cell wall polysaccharide, 
      peptidoglycan, carbohydrates and proteins plays an important role in adsorption 
      of AO, also involving hydrophilic and ionic interactions. Binding, 
      biotransformation and detoxification of MNNG and MeIQx by Lr 231 was dependent on 
      cell surface characteristics mainly involving carbohydrates, proteins, teichoic 
      acid/lipoteichoic acid, hydrophobic interaction and presence of thiol group. L. 
      rhamnosus 231 bound MNNG instantaneously. More than 96 (p < 0.01) and 70% (p < 
      0.05) cells remained viable after mutagen binding and various pretreatments 
      respectively. This study shows Lr 231 exhibits ability to bind and detoxify 
      potent mutagens, and this property can be useful in formulating fermented foods 
      for removal of potent mutagens.
CI  - Copyright (c) 2011 Elsevier Ltd. All rights reserved.
FAU - Ambalam, Padma
AU  - Ambalam P
AD  - Department of Biosciences, Saurashtra University, Rajkot, India.
FAU - Dave, J M
AU  - Dave JM
FAU - Nair, Baboo M
AU  - Nair BM
FAU - Vyas, B R M
AU  - Vyas BR
LA  - eng
PT  - Journal Article
DEP - 20110712
PL  - England
TA  - Anaerobe
JT  - Anaerobe
JID - 9505216
RN  - 0 (Antimutagenic Agents)
RN  - 0 (Culture Media)
RN  - 0 (Mutagens)
RN  - 0 (Phenylenediamines)
RN  - 0 (Quinoxalines)
RN  - 12H3O2UGSF (Methylnitronitrosoguanidine)
RN  - 5A9AX7Y0TT (1,2-diamino-4-nitrobenzene)
RN  - 77500-04-0 (2-amino-3,8-dimethylimidazo(4,5-f)quinoxaline)
RN  - F30N4O6XVV (Acridine Orange)
SB  - IM
MH  - Acridine Orange/chemistry/metabolism
MH  - *Antimutagenic Agents
MH  - Biotransformation
MH  - Culture Media
MH  - Feces/microbiology
MH  - Humans
MH  - Hydrogen-Ion Concentration
MH  - Lacticaseibacillus rhamnosus/growth & development/isolation & 
      purification/*metabolism
MH  - Methylnitronitrosoguanidine/chemistry/metabolism
MH  - Microbial Viability
MH  - Mutagens/chemistry/*metabolism
MH  - Phenylenediamines/chemistry/metabolism
MH  - Quinoxalines/chemistry/metabolism
EDAT- 2011/07/23 06:00
MHDA- 2012/01/12 06:00
CRDT- 2011/07/23 06:00
PHST- 2011/02/24 00:00 [received]
PHST- 2011/06/20 00:00 [revised]
PHST- 2011/07/01 00:00 [accepted]
PHST- 2011/07/23 06:00 [entrez]
PHST- 2011/07/23 06:00 [pubmed]
PHST- 2012/01/12 06:00 [medline]
AID - S1075-9964(11)00131-4 [pii]
AID - 10.1016/j.anaerobe.2011.07.001 [doi]
PST - ppublish
SO  - Anaerobe. 2011 Oct;17(5):217-22. doi: 10.1016/j.anaerobe.2011.07.001. Epub 2011 
      Jul 12.