PMID- 2177773 OWN - NLM STAT- MEDLINE DCOM- 19910314 LR - 20190828 IS - 0146-6615 (Print) IS - 0146-6615 (Linking) VI - 32 IP - 2 DP - 1990 Oct TI - Enzyme-linked immunosorbent assay for detection of IgG antibody to human herpesvirus 6. PG - 119-23 AB - A lysate of human herpesvirus 6 (HHV-6)-infected cord blood mononuclear cells was used as antigen for enzyme-linked immunosorbent assay for the detection of IgG antibody to HHV-6. Antibody responses after exanthem subitum were well correlated with clinical recovery from the disease and the level of antibody activities was well correlated with indirect immunofluorescence assay and the neutralization test. Seroconversion to other human herpesvirus, including cytomegalovirus, was not observed in infants with exanthem subitum. All of the infants had by the age of 1 month antibodies to HHV-6, which decreased with age to the lowest level at the age of 3 to 6 months and then increased and reached the maximum level by 1 to 2 years of age. After 3 years of age, the prevalence was almost stable. FAU - Asano, Y AU - Asano Y AD - Department of Pediatrics, Fujita Health University School of Medicine, Aichi, Japan. FAU - Yoshikawa, T AU - Yoshikawa T FAU - Suga, S AU - Suga S FAU - Yazaki, T AU - Yazaki T FAU - Ozaki, T AU - Ozaki T FAU - Saito, Y AU - Saito Y FAU - Hatano, Y AU - Hatano Y FAU - Takahashi, M AU - Takahashi M LA - eng PT - Comparative Study PT - Journal Article PL - United States TA - J Med Virol JT - Journal of medical virology JID - 7705876 RN - 0 (Antibodies, Viral) RN - 0 (Immunoglobulin G) SB - IM MH - Adolescent MH - Adult MH - Antibodies, Viral/*analysis MH - Child MH - Child, Preschool MH - Cross Reactions MH - Enzyme-Linked Immunosorbent Assay MH - Exanthema Subitum/complications/epidemiology/*immunology MH - Female MH - Fluorescent Antibody Technique MH - Herpesviridae Infections/epidemiology/*immunology MH - Herpesvirus 6, Human/*immunology MH - Humans MH - Immunoglobulin G/*immunology MH - Infant MH - Infant, Newborn MH - Male MH - Middle Aged MH - Neutralization Tests MH - Reproducibility of Results MH - Seroepidemiologic Studies EDAT- 1990/10/01 00:00 MHDA- 1990/10/01 00:01 CRDT- 1990/10/01 00:00 PHST- 1990/10/01 00:00 [pubmed] PHST- 1990/10/01 00:01 [medline] PHST- 1990/10/01 00:00 [entrez] AID - 10.1002/jmv.1890320209 [doi] PST - ppublish SO - J Med Virol. 1990 Oct;32(2):119-23. doi: 10.1002/jmv.1890320209.