PMID- 2178012
OWN - NLM
STAT- MEDLINE
DCOM- 19910315
LR  - 20190510
IS  - 0007-1188 (Print)
IS  - 0007-1188 (Linking)
VI  - 101
IP  - 1
DP  - 1990 Sep
TI  - Differential regulation of 5-lipoxygenase and cyclo-oxygenase pathways of 
      arachidonate metabolism in rat peritoneal leukocytes.
PG  - 128-32
AB  - 1. Simultaneous activation of the 5-lipoxygenase and cyclo-oxygenase pathways of 
      arachidonate metabolism in rat peritoneal mixed leukocytes in response to A23187, 
      chemoattractant N-formyl-methionine-leucine-phenylalanine (FMLP) and arachidonic 
      acid (AA) was studied by radioimmunoassay of leukotriene B4 (LTB4) and 
      thromboxane B2 (TXB2) respectively. 2. FMLP and AA preferentially activated 
      cyclo-oxygenase and A23187 preferentially activated 5-lipoxygenase. Release of 
      TXB2 preceded that of LTB4. A threshold amount of A23187 enhanced FMLP-and 
      AA-induced LTB4 production but not that of TXB2. 3. Phorbol myristate acetate 
      (PMA) abolished LTB4 generation in response to FMLP with much less effect on 
      TXB2, but did not inhibit the formation of either eicosanoid caused by A23187 or 
      AA. Instead, PMA caused a dose-dependent but modest stimulation of TXB2 
      generation either on its own or when added with A23187 or AA. 4. These results 
      show that the 5-lipoxygenase and cyclo-oxygenase pathways in rat peritoneal 
      leukocytes are regulated differently and that functional compartmentalisation of 
      the stimulus-generation sequence operates in these cells.
FAU - Moroney, M A
AU  - Moroney MA
AD  - King's College London.
FAU - Forder, R A
AU  - Forder RA
FAU - Carey, F
AU  - Carey F
FAU - Hoult, J R
AU  - Hoult JR
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Br J Pharmacol
JT  - British journal of pharmacology
JID - 7502536
RN  - 0 (Arachidonic Acids)
RN  - 1HGW4DR56D (Leukotriene B4)
RN  - 27YG812J1I (Arachidonic Acid)
RN  - 37H9VM9WZL (Calcimycin)
RN  - 54397-85-2 (Thromboxane B2)
RN  - 59880-97-6 (N-Formylmethionine Leucyl-Phenylalanine)
RN  - EC 1.13.11.34 (Arachidonate 5-Lipoxygenase)
RN  - EC 1.14.99.1 (Prostaglandin-Endoperoxide Synthases)
RN  - NI40JAQ945 (Tetradecanoylphorbol Acetate)
SB  - IM
MH  - Animals
MH  - Arachidonate 5-Lipoxygenase/*metabolism
MH  - Arachidonic Acid
MH  - Arachidonic Acids/*metabolism
MH  - Calcimycin/pharmacology
MH  - Female
MH  - In Vitro Techniques
MH  - Leukocytes/drug effects/*enzymology
MH  - Leukotriene B4/pharmacology
MH  - N-Formylmethionine Leucyl-Phenylalanine/pharmacology
MH  - Peritoneal Cavity/cytology
MH  - Prostaglandin-Endoperoxide Synthases/*metabolism
MH  - Radioimmunoassay
MH  - Rats
MH  - Tetradecanoylphorbol Acetate/pharmacology
MH  - Thromboxane B2/pharmacology
PMC - PMC1917650
EDAT- 1990/09/01 00:00
MHDA- 1990/09/01 00:01
PMCR- 1991/09/01
CRDT- 1990/09/01 00:00
PHST- 1990/09/01 00:00 [pubmed]
PHST- 1990/09/01 00:01 [medline]
PHST- 1990/09/01 00:00 [entrez]
PHST- 1991/09/01 00:00 [pmc-release]
AID - 10.1111/j.1476-5381.1990.tb12101.x [doi]
PST - ppublish
SO  - Br J Pharmacol. 1990 Sep;101(1):128-32. doi: 10.1111/j.1476-5381.1990.tb12101.x.