PMID- 21780950
OWN - NLM
STAT- MEDLINE
DCOM- 20120430
LR  - 20151119
IS  - 1473-0766 (Electronic)
IS  - 0951-3590 (Linking)
VI  - 28
IP  - 1
DP  - 2012 Jan
TI  - Establishment of polycystic ovary syndrome-derived human embryonic stem cell 
      lines.
PG  - 25-8
LID - 10.3109/09513590.2011.588748 [doi]
AB  - BACKGROUND: Recently, human embryonic stem cells (hESCs) of some genetic diseases 
      have been established, but little research has been done on polycystic ovary 
      syndrome (PCOS)-derived hESCs. The establishment of PCOS-derived hESCs provides a 
      biological basis for exploring the pathogenesis, gene mapping and gene therapy of 
      PCOS. METHODS: Discarded fresh embryos were collected and cultured until the 
      blastocyst stage, and then inner cell masses (ICM) were isolated by mechanical 
      methods and incubated in the mixed feeder layer containing human stem cell 
      medium. hESCs were identified whether to maintain normal karyotype and 
      pluripotency by alkaline phosphates (AKP), stage-specific embryonic antigen-4 
      (SSEA-4), NANOG, SOX2 and TRA-1-60, octamer binding protein 4(OCT-4), and in vivo 
      and in vitro differentiation. RESULTS: Of the 11 passaged ICM, nine showed 
      adherent growths within 48 h with an adherence rate of 81.8% (9/11). Five 
      PCOS-derived hESCs were established and all of them have the characteristics of 
      pluripotent differentiation. One was from 2PN embryo which was retarded in the 
      cleavage stage, one was from 1PN embryo and others were from 0PN embryo. They 
      were named p-hES-1, p-hES-2, p-hES-3, p-hES-4, p-hES-5, respectively. CONCLUSION: 
      We provide biological models for studying the pathogenesiss of PCOS.
FAU - Li, Peng-fen
AU  - Li PF
AD  - Reproductive Medical Center, Department of Obstetrics and Gynecology, First 
      Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China.
FAU - Wang, Fang
AU  - Wang F
FAU - Kong, Hui-juan
AU  - Kong HJ
FAU - Zhao, Fang
AU  - Zhao F
FAU - Bai, Ai-hong
AU  - Bai AH
FAU - Chen, Xue-mei
AU  - Chen XM
FAU - Sun, Ying-pu
AU  - Sun YP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110723
PL  - England
TA  - Gynecol Endocrinol
JT  - Gynecological endocrinology : the official journal of the International Society 
      of Gynecological Endocrinology
JID - 8807913
RN  - 0 (Biomarkers)
SB  - IM
MH  - Biomarkers/analysis/metabolism
MH  - Blastocyst/cytology/metabolism/pathology
MH  - Cell Line
MH  - Cell Separation
MH  - Embryo Disposition
MH  - Embryo, Mammalian
MH  - Embryonic Stem Cells/metabolism/*pathology
MH  - Feeder Cells/cytology
MH  - Female
MH  - Fertilization in Vitro
MH  - Foreskin/cytology
MH  - Humans
MH  - Male
MH  - Polycystic Ovary Syndrome/genetics/metabolism/*pathology
MH  - Primary Cell Culture/*methods
MH  - Reverse Transcriptase Polymerase Chain Reaction
EDAT- 2011/07/26 06:00
MHDA- 2012/05/01 06:00
CRDT- 2011/07/26 06:00
PHST- 2011/07/26 06:00 [entrez]
PHST- 2011/07/26 06:00 [pubmed]
PHST- 2012/05/01 06:00 [medline]
AID - 10.3109/09513590.2011.588748 [doi]
PST - ppublish
SO  - Gynecol Endocrinol. 2012 Jan;28(1):25-8. doi: 10.3109/09513590.2011.588748. Epub 
      2011 Jul 23.