PMID- 21782513
OWN - NLM
STAT- MEDLINE
DCOM- 20120112
LR  - 20110905
IS  - 1472-6491 (Electronic)
IS  - 1472-6483 (Linking)
VI  - 23
IP  - 3
DP  - 2011 Sep
TI  - Seven years of experience of preimplantation HLA typing: a clinical overview of 
      327 cycles.
PG  - 363-71
LID - 10.1016/j.rbmo.2011.05.016 [doi]
AB  - Preimplantation human leukocyte antigen (HLA) typing allows the birth of healthy 
      children who are potential donors of stem cells for their affected siblings. This 
      technique can be used for acquired diseases such as leukaemia or can be used for 
      single-gene disorders such as thalassaemia. This retrospective study presents 
      clinical data obtained from 171 couples who had undergone 327 preimplantation HLA 
      typing cycles: 262 cycles for HLA typing in combination with mutation analysis 
      and 65 cycles for the sole purpose of HLA typing. Of the diagnosed embryos 17.6% 
      were found to be HLA matched. Embryo transfer was performed in 212 cycles, 34.9% 
      clinical pregnancy rate per transfer was achieved and 59 healthy and 
      HLA-compatible children were born. Twenty-one sick children have been cured 
      through haemopoietic stem cell transplantation. The effect of maternal age and 
      ovarian reserve on reproductive outcome was assessed retrospectively. The data 
      demonstrated that, once a mutation-free and HLA-compatible embryo was found, 
      clinical pregnancy rate did not differ statistically significantly despite the 
      presence of some cycle-related limitations such as advanced maternal age and/or 
      diminished ovarian reserve. Preimplantation HLA typing is an effective 
      therapeutic tool for curing an affected sibling even for poor-prognosis patients. 
      Preimplantation human leukocyte antigent (HLA) typing allows the birth of healthy 
      children who are potential donors of stem cells for their affected siblings. This 
      technique can be used for acquired diseases such as leukaemia or can be used for 
      single-gene disorders such as thalassaemia. This study presents clinical data 
      obtained from 171 couples who underwent 327 preimplantation HLA-typing cycles. Of 
      these, 262 cycles were performed for HLA typing in combination with mutation 
      analysis and 65 cycles were performed for the sole purpose of HLA typing. A total 
      of 17.6% of the diagnosed embryos were found to be HLA matched. Embryo transfer 
      was performed in 212 cycles. The clinical pregnancy rate per transfer was 34.9% 
      and 59 healthy and HLA compatible children were born. Twenty-one sick children 
      have been cured through haemopoietic stem cell transplantation. The effect of 
      maternal age and ovarian reserve on reproductive outcome was assessed 
      retrospectively. The data demonstrated that, once a mutation-free and 
      HLA-compatible embryo was found, clinical pregnancy rates did not differ 
      statistically significantly by the presence of some cycle-related limitations 
      such as advanced maternal age and/or diminished ovarian reserve. Preimplantation 
      HLA typing is an effective therapeutic tool for curing an affected sibling even 
      for poor-prognosis patients.
CI  - Copyright (c) 2011 Reproductive Healthcare Ltd. Published by Elsevier Ltd. All 
      rights reserved.
FAU - Kahraman, Semra
AU  - Kahraman S
AD  - In Vitro Fertilisation Unit, Istanbul Memorial Hospital, Piyalepasa Bulvari, 
      Sisli, Istanbul, Turkey.
FAU - Beyazyurek, Cagri
AU  - Beyazyurek C
FAU - Ekmekci, Cumhur Gokhan
AU  - Ekmekci CG
LA  - eng
PT  - Journal Article
DEP - 20110615
PL  - Netherlands
TA  - Reprod Biomed Online
JT  - Reproductive biomedicine online
JID - 101122473
SB  - IM
CIN - Reprod Biomed Online. 2011 Sep;23(3):271-3. PMID: 21802364
MH  - Blastocyst/*immunology
MH  - Embryo Transfer
MH  - Female
MH  - Fertilization in Vitro
MH  - *Histocompatibility Testing
MH  - Humans
MH  - Pregnancy
MH  - Pregnancy Rate
MH  - Preimplantation Diagnosis
MH  - Retrospective Studies
MH  - Siblings
EDAT- 2011/07/26 06:00
MHDA- 2012/01/13 06:00
CRDT- 2011/07/26 06:00
PHST- 2010/11/08 00:00 [received]
PHST- 2011/04/29 00:00 [revised]
PHST- 2011/05/11 00:00 [accepted]
PHST- 2011/07/26 06:00 [entrez]
PHST- 2011/07/26 06:00 [pubmed]
PHST- 2012/01/13 06:00 [medline]
AID - S1472-6483(11)00324-5 [pii]
AID - 10.1016/j.rbmo.2011.05.016 [doi]
PST - ppublish
SO  - Reprod Biomed Online. 2011 Sep;23(3):363-71. doi: 10.1016/j.rbmo.2011.05.016. 
      Epub 2011 Jun 15.