PMID- 21782719
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110725
IS  - 1382-6689 (Print)
IS  - 1382-6689 (Linking)
VI  - 17
IP  - 2
DP  - 2004 Jun
TI  - Evaluation of lymphocyte subpopulations in draining lymph node cells following 
      allergen and irritant.
PG  - 95-102
LID - 10.1016/j.etap.2004.03.006 [doi]
AB  - The murine local lymph node assay (LLNA) has been developed as an alternative to 
      guinea pig models for the assessment of the contact sensitization potential. 
      However, there is a need to develop a non-radioisotopic endpoint for the LLNA, 
      because of the radioisotopic method's requiring the use of special facilities. In 
      this study, we investigated to evaluate the lymphocyte subpopulations in the 
      lymph node cells following allergen and irritant treatment. Female Balb/c mice 
      were treated by the topical application on the dorsum of both ears with 
      sensitizers, 2,4-dinitrochlorobenzene (DNCB), toluene diisocyanate (TDI), and 
      alpha-hexylcinnamaldehyde (HCA), and an irritant, sodium lauryl sulfate (SLS), once 
      daily for three consecutive days. The lymph node (LN) cells were harvested 72h 
      after the final treatment. Phenotypic analysis of lymphocytes subsets was 
      performed with a flow cytometry. The allergens DNCB, TDI, and HCA and an 
      irritant, SLS increased cell number compared to the vehicle. Mice were treated 
      with DNCB, HCA, and TDI showed a preferential increase in the percentage of 
      B220+CD40+ cells compared with vehicle and irritant-treated mice. There was an 
      increase in B220+CD86+ cells of mice treated with DNCB, TDI, and HCA, but no 
      significant increases were observed in mice treated with SLS. Mice were treated 
      with DNCB and TDI showed an increase in the percentage of B220+CD23+ cells 
      compared with vehicle and irritant-treated mice. These results suggest that 
      analysis of B cell activation marker, CD40 on B cells may be useful in 
      differentiating allergen and irritant responses in the draining lymph nodes of 
      chemically treated mice.
FAU - Lee, Jong Kwon
AU  - Lee JK
AD  - Division of Immunotoxicology, National Institute of Toxicology Research, Korea 
      Food and Drug Administration, 122-704 Seoul, South Korea.
FAU - Hee Park, Seung
AU  - Hee Park S
FAU - Byun, Jung A
AU  - Byun JA
FAU - Kim, Hyung Soo
AU  - Kim HS
FAU - Oh, Hye Young
AU  - Oh HY
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
EDAT- 2004/06/01 00:00
MHDA- 2004/06/01 00:01
CRDT- 2011/07/26 06:00
PHST- 2004/01/12 00:00 [received]
PHST- 2004/03/24 00:00 [accepted]
PHST- 2004/01/12 00:00 [received]
PHST- 2004/03/24 00:00 [accepted]
PHST- 2011/07/26 06:00 [entrez]
PHST- 2004/06/01 00:00 [pubmed]
PHST- 2004/06/01 00:01 [medline]
AID - S1382-6689(04)00064-X [pii]
AID - 10.1016/j.etap.2004.03.006 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2004 Jun;17(2):95-102. doi: 
      10.1016/j.etap.2004.03.006.