PMID- 21782801
OWN - NLM
STAT- MEDLINE
DCOM- 20111014
LR  - 20161125
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 411
IP  - 4
DP  - 2011 Aug 12
TI  - Degradation of the GAB1 adaptor by the ubiquitin-proteasome pathway hampers 
      HGF/SF-MET signaling.
PG  - 780-5
LID - 10.1016/j.bbrc.2011.07.024 [doi]
AB  - The GRB2 associated binder 1 (GAB1) is an essential docking/adaptor protein for 
      transmitting intracellular signals of the MET tyrosine kinase receptor activated 
      by hepatocyte growth factor/scatter factor (HGF/SF). We found that in response to 
      hours of HGF/SF treatment, the GAB1 protein level is degraded by a mechanism 
      involving MET activity and the proteasomal machinery. We also showed that GAB1 is 
      both multi- and poly-ubiquitinated in a CBL-dependent manner. A long term 
      exposure to HGF/SF caused a more sustained down-regulation of GAB1 than of MET, 
      associated with a loss of reactivation of the ERK MAP kinases to subsequent acute 
      ligand treatment. These data demonstrate that GAB1 is ubiquitinated by CBL and 
      degraded by the proteasome, and plays a role in negative-feedback regulation of 
      HGF/SF-MET signaling.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Goormachtigh, Gautier
AU  - Goormachtigh G
AD  - CNRS UMR, Institut de Biologie de Lille, Universite Lille-Nord de France, 
      Institut Pasteur de Lille, France.
FAU - Ji, Zongling
AU  - Ji Z
FAU - Le Goff, Arnaud
AU  - Le Goff A
FAU - Fafeur, Veronique
AU  - Fafeur V
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20110718
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (GAB1 protein, human)
RN  - 0 (HGF protein, human)
RN  - 0 (Receptors, Growth Factor)
RN  - 0 (Ubiquitin)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - EC 2.3.2.27 (Proto-Oncogene Proteins c-cbl)
RN  - EC 2.7.10.1 (MET protein, human)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 3.4.25.1 (Proteasome Endopeptidase Complex)
RN  - EC 6.3.2.- (CBL protein, human)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing/*metabolism
MH  - HEK293 Cells
MH  - HeLa Cells
MH  - Hepatocyte Growth Factor/*metabolism
MH  - Humans
MH  - Proteasome Endopeptidase Complex/*metabolism
MH  - Proto-Oncogene Proteins c-cbl/metabolism
MH  - Proto-Oncogene Proteins c-met/*metabolism
MH  - Receptors, Growth Factor/*metabolism
MH  - Signal Transduction
MH  - Ubiquitin/metabolism
MH  - *Ubiquitination
EDAT- 2011/07/26 06:00
MHDA- 2011/10/15 06:00
CRDT- 2011/07/26 06:00
PHST- 2011/07/04 00:00 [received]
PHST- 2011/07/06 00:00 [accepted]
PHST- 2011/07/26 06:00 [entrez]
PHST- 2011/07/26 06:00 [pubmed]
PHST- 2011/10/15 06:00 [medline]
AID - S0006-291X(11)01243-5 [pii]
AID - 10.1016/j.bbrc.2011.07.024 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2011 Aug 12;411(4):780-5. doi: 
      10.1016/j.bbrc.2011.07.024. Epub 2011 Jul 18.