PMID- 21783466
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110725
IS  - 1382-6689 (Print)
IS  - 1382-6689 (Linking)
VI  - 19
IP  - 1
DP  - 2005 Jan
TI  - Genotoxicity evaluation of metronidazole using the piscine micronucleus test by 
      acridine orange fluorescent staining.
PG  - 107-11
LID - 10.1016/j.etap.2004.05.007 [doi]
AB  - In this study, genotoxic potential of metronidazole, an antibiotic-antiparasitic 
      agent widely used both clinical and veterinary areas, was evaluated using the 
      piscine micronucleus test. Specimens of Oreochromis niloticus (Pisces: Cichlidae) 
      were exposed to different concentrations (5, 10, 15mg/L) of metronidazole and 
      4mg/L cyclophosphamide (as positive control) for 24, 48 and 72h. A fluorescent 
      staining technique with acridine orange (AO) was performed to improve the 
      sensitivity of erythrocyte micronucleus assay with fish. AO selectively stains 
      newly formed immature erythrocytes (PCEs) therefore allows to obtain more 
      sensitive results by detection of the micronucleated PCEs differed than mature 
      erythrocytes (NCEs) by their RNA-containing cytoplasm. The ratio of PCE/NCE in 
      peripheral blood was also evaluated to assess cytotoxicity. As a result, it was 
      observed that the frequencies of micronucleated PCEs increased both dose and time 
      dependently while PCE/NCE ratios decreased. Our results revealed that 
      metronidazole has cytotoxic and genotoxic effects on fish. The use of acridine 
      orange staining technique also seems useful in assessment of short-term genotoxic 
      effects of chemicals, when fish are used as experimental animal.
FAU - Cavas, Tolga
AU  - Cavas T
AD  - Department of Biology, Faculty of Sciences and Letters, Mersin University, 33342 
      Mersin, Turkey.
FAU - Ergene-Gozukara, Serap
AU  - Ergene-Gozukara S
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
EDAT- 2005/01/01 00:00
MHDA- 2005/01/01 00:01
CRDT- 2011/07/26 06:00
PHST- 2004/01/12 00:00 [received]
PHST- 2004/05/25 00:00 [accepted]
PHST- 2004/01/12 00:00 [received]
PHST- 2004/05/25 00:00 [accepted]
PHST- 2011/07/26 06:00 [entrez]
PHST- 2005/01/01 00:00 [pubmed]
PHST- 2005/01/01 00:01 [medline]
AID - S1382-6689(04)00090-0 [pii]
AID - 10.1016/j.etap.2004.05.007 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2005 Jan;19(1):107-11. doi: 
      10.1016/j.etap.2004.05.007.