PMID- 21783593
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110725
IS  - 1382-6689 (Print)
IS  - 1382-6689 (Linking)
VI  - 20
IP  - 1
DP  - 2005 Jul
TI  - Modulation of intracellular cytokines in draining lymph node cells following 
      allergen and irritant.
PG  - 225-32
LID - 10.1016/j.etap.2005.02.002 [doi]
AB  - The murine local lymph node assay (LLNA) has been developed as an alternative to 
      guinea pig models for the assessment of the contact sensitization potential. 
      However, there is a need to develop a non-radioisotopic endpoint for the LLNA 
      because of the radioisotopic method's requiring the use of special facilities. In 
      this study, we investigated to evaluate the populations of intracellular cytokine 
      producing cells and to analyze the expression of mRNA levels in the lymph node 
      (LN) cells following allergen and irritant. Female Balb/c mice were treated by 
      the topical application on the dorsum of both ears with strong sensitizers, 
      2,4-dinitrochlorobenzene (DNCB) and toluene diisocyanate (TDI) and a strong 
      irritant, sodium lauryl sulfate (SLS), once daily for 3 consecutive days. The 
      lymph node cells were harvested 72h after the final treatment. The analysis of 
      intracellular cytokine cell in LN cells was performed with a flow cytometry. Mice 
      were treated with DNCB and TDI showed a preferential increase in the percentage 
      of CD4+IL-2+ cells compared with vehicle and irritant-treated mice. There was an 
      increase in CD4+IFN-g+ cells of mice treated with DNCB and TDI, but no 
      significant increases were observed in mice treated with SLS. Mice were treated 
      with DNCB and TDI showed an increase in the percentage of CD4+IL-4+ cells 
      compared with vehicle and irritant-treated mice. There was an increase in the 
      mRNA level for interleukin 4 (IL-4) in mice treated with DNCB and TDI, but no 
      significant increases were observed in mice treated with SLS. These results 
      suggest that the population of interferon-gamma (IFN-g+) and IL-4+ cells on CD4+ 
      cells and the mRNA expression for IL-4 in lymphocytes could be selectively 
      modulated in allergen-treated mice.
FAU - Lee, Jong Kwon
AU  - Lee JK
AD  - Division of Immunotoxicology, National Institute of Toxicology Research, Korea 
      Food and Drug Administration, 122-704 Seoul, South Korea.
FAU - Park, Jae Hyun
AU  - Park JH
FAU - Eom, Juno H
AU  - Eom JH
FAU - Kim, Hyung Soo
AU  - Kim HS
FAU - Oh, Hye Young
AU  - Oh HY
LA  - eng
PT  - Journal Article
DEP - 20050323
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
EDAT- 2005/07/01 00:00
MHDA- 2005/07/01 00:01
CRDT- 2011/07/26 06:00
PHST- 2004/10/10 00:00 [received]
PHST- 2005/02/10 00:00 [accepted]
PHST- 2011/07/26 06:00 [entrez]
PHST- 2005/07/01 00:00 [pubmed]
PHST- 2005/07/01 00:01 [medline]
AID - S1382-6689(05)00020-7 [pii]
AID - 10.1016/j.etap.2005.02.002 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2005 Jul;20(1):225-32. doi: 
      10.1016/j.etap.2005.02.002. Epub 2005 Mar 23.