PMID- 21783968
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20110725
IS  - 1382-6689 (Print)
IS  - 1382-6689 (Linking)
VI  - 27
IP  - 3
DP  - 2009 May
TI  - Reduction of cyclophosphamide-induced DNA damage and apoptosis effects of 
      ginsenoside Rb(1) on mouse bone marrow cells and peripheral blood leukocytes.
PG  - 384-9
LID - 10.1016/j.etap.2009.01.001 [doi]
AB  - The present study investigated the protective effects of ginsenoside Rb(1) 
      (GRb(1)) against genotoxicity induced by cyclophosphamide (CP). Single cell gel 
      electrophoresis, flow cytometry assay with annexin V-FITC/propidine iodide (PI) 
      and acridine orange (AO)/ethidium bromide (EB) staining assay were employed to 
      measure DNA damage and cell apoptosis, respectively. The activities of total 
      superoxide dismutase (T-SOD) and glutathione peroxidase (GPx) and the 
      malondialdehyde (MDA) content were also investigated by a number of colormetric 
      methods. The results showed that the CP produced significant DNA damage and cell 
      apoptosis in mouse bone marrow cells or peripheral blood leukocytes, markedly 
      inhibited the activities of T-SOD and GPx, and markedly increased the MDA 
      content. GRb(1) significantly inhibited DNA damages and cell apoptosis in mouse 
      bone marrow cells or peripheral blood leukocytes induced by CP and antagonized 
      the reduction of CP-induced T-SOD and GPx activities, and inhibited the increase 
      in MDA content induced by CP. The anti-tumor study of GRb(1) showed that GRb(1) 
      did not affect the anti-tumor activities of CP. In conclusion, GRb(1) had 
      significant protective effects against DNA damage and apoptosis induced by CP.
CI  - Copyright (c) 2009 Elsevier B.V. All rights reserved.
FAU - Zhang, Qiu Hua
AU  - Zhang QH
AD  - Department of Basic Pharmacology, Liaoning University of Traditional Chinese 
      Medicine,110032 Shenyang, PR China; Department of Pharmacology, Shenyang 
      Pharmaceutical University, Shenyang 110016, PR China.
FAU - Wu, Chun Fu
AU  - Wu CF
FAU - Yang, Jing Yu
AU  - Yang JY
FAU - Mu, Yan Hua
AU  - Mu YH
FAU - Chen, Xiao Xue
AU  - Chen XX
FAU - Zhao, Yu Qing
AU  - Zhao YQ
LA  - eng
PT  - Journal Article
DEP - 20090115
PL  - Netherlands
TA  - Environ Toxicol Pharmacol
JT  - Environmental toxicology and pharmacology
JID - 9612020
EDAT- 2009/05/01 00:00
MHDA- 2009/05/01 00:01
CRDT- 2011/07/26 06:00
PHST- 2008/01/09 00:00 [received]
PHST- 2008/12/24 00:00 [revised]
PHST- 2009/01/07 00:00 [accepted]
PHST- 2011/07/26 06:00 [entrez]
PHST- 2009/05/01 00:00 [pubmed]
PHST- 2009/05/01 00:01 [medline]
AID - S1382-6689(09)00003-9 [pii]
AID - 10.1016/j.etap.2009.01.001 [doi]
PST - ppublish
SO  - Environ Toxicol Pharmacol. 2009 May;27(3):384-9. doi: 10.1016/j.etap.2009.01.001. 
      Epub 2009 Jan 15.